大鼠烫伤后早期转化生长因子β1/Smads信号通路的活化
摘要
1材料与方法
1.1主要试剂来源 一抗:小鼠抗大鼠β肌动蛋白单克隆抗体(美国Sigma公司),山羊抗大鼠磷酸化Smad2/3多克隆抗体、兔抗大鼠Smad3多克隆抗体(美国Santa Cruz公司);二抗:辣根过氧化物酶(HRP)标记的驴抗兔或山羊多克隆抗体(美国Santa Cruz公司),HRP标记的山羊抗小鼠多克隆抗体(美国Calbioehem公司)。SB431542(美国Tocris公司),Trizol试剂(美国GibeoBRL公司),反转录(RT)-PCR试剂盒(大连宝生物工程有限公司),二辛丁酸(BCA)试剂盒、肿瘤坏死因子仅(TNF-α)酶联免疫吸附测定(ELISA)试剂盒及白细胞介素1β(IL-1β)ELISA试剂盒(美国Pierce公司),IL-6ELISA试剂盒(美国R&D公司)。PCR仪(美国Biometa公司),半干电转移仪、图像分析仪、550型酶标仪(美国Bio—Rad公司)。
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2008年第1期55-56,共2页
Chinese Journal of Burns
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共引文献3
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1周洋,金一帮,王俊华,吐尔洪江·吐逊,魏晓丽,张志,张传山,李亮,林仁勇,温浩.细粒棘球蚴囊液对体外培养小鼠脾细胞Foxp3及Smad4基因表达的影响[J].中国病原生物学杂志,2011,6(3):193-196. 被引量:7
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