摘要
目的探讨高脂饮食所致大鼠非酒精性脂肪性肝病(NAFLD)发生发展中血清脂联素(ADP)水平的变化及作用机制。方法通过高脂饮食建立大鼠非酒精性脂肪性肝病模型,并在第8,10,12周末分批处死大鼠,同期设正常饮食组作为对照组。检测血清ADP、肿瘤坏死因子α(TNF-α)、谷丙转氨酶(ALT)及内毒素(ET)等指标,并计算胰岛素抵抗指数(IRI);肝脏HE染色,观察肝脏的形态学变化。结果模型组大鼠第8周呈现单纯性脂肪肝,第10-12周进展为脂肪性肝炎。与对照组比较,血清ADP水平8周起明显降低(P<0.01),同时血清TNF-α、ET及IRI明显升高(P<0.05),TNF-α、IRI均与脂联素呈高度负相关(r=-0.673,-0.787,P<0.01);10-12周血清ALT明显升高(P<0.05)。结论IR可能是NAFLD发生、发展的基础,NAFLD大鼠血清脂联素降低,从而通过一定机制参与了IR及炎症反应,进而影响了NAFLD的发生、发展。
Objective To investigate the changes of plasma adiponectin of rats with nonalcoholic fatty liver disease （NAFLD） chronically fed with a fat-riched diet and its mechanism. Methods Thirty-six male Wistar rats were randomly divided into 2 groups： control group（normal diet） and model group（high fat-fed）. All rats were sacrificed at the end of 8, 10, 12 weeks. The blood of abdominal aorta was obtained and the levels of ET, ALT, TNF-α and ADP in liver were measured. And IRI was calculated. The changes of hepatic morphology were observed by HE. Results Simple fatty liver was observed in model group at 8 week. From week 10 to week 12, the liver gradually developed steatohepatitis. Compared with control group, the levels of adiponectin in model group decreased significantly at 8 week （P 〈 0.01）, while the levels of TNF-α, ET, and IRI increased significantly （P 〈 0.01）. From week 10 to week 12, ALT levels increased （P 〈 0.05）. TNF-α, IRI were negatively related to adiponectin （r = -0. 673, -0. 787, P〈 0.01）. Conclusion Insulin resistance plays an important role in high fat-fed induced fatty liver. Plasma adiponectin may participate in insulin resistance and inflammation, and influence the development of NAFLD.
出处
《山西医科大学学报》
CAS
2008年第2期133-135,共3页
Journal of Shanxi Medical University
基金
山西省科技农业公关(2007031092-1)
山西省卫生厅卫生科技发展计划项目(200305)
关键词
脂肪肝
非酒精性
胰岛素抵抗
脂联素
fatty liver, non-alcoholic
insulin resistance
adiponectin
