摘要
利用激光扫描共聚焦显微镜（Confocal microscope）采用pH依赖的荧光探针Snarf-4F测量细胞胞内pH（pHi）方法，发现胞外ATP能剂量依赖（10—1000μmol／L）地降低人阴道上皮细胞株VK2／E6E7细胞pHi。当胞外加入200μmol／L ATP时，能快速地使VK2／E6E7细胞pHi降低，此降低的pHi在洗脱ATP后可迅速恢复。这些结果表明胞外ATP能引起VK2／E6E7细胞胞内酸化。
Using Confocal microscope system in human vaginal epithelial cell line VK2/E6E7 cells loaded with the pH-dependent fluorescent probe Snarf-4F, external ATP （ 10 - 1 000 μmol/L） was proved to dose-dependently induce phi decrease. When adding ATP （200 μmol/L） to the extracellular buffer, it induced a rapid pHi decrease and the intracellular acidification was rapidly reversed upon removal of external ATP. These results show external ATP could induce the intracellular acidification of VK2/E6E7 cells.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第1期123-124,共2页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金资助项目(30570229,30770817)
