摘要
目的:研究肿瘤坏死因子相关凋亡诱导配体(TNF-related apoptosis inducing ligand,TRAIL)受体在正常子宫内膜组织、子宫内膜癌组织和癌旁组织的表达特征。方法:采用RT-PCR技术,检测20例正常子宫内膜、20例子宫内膜癌及癌旁组织中TRAIL受体DR4、DR5和假受体DcR1、DcR2阳性表达情况。结果:TRAIL受体DR4、DR5的mRNA在人正常子宫内膜组织、子宫内膜癌组织和癌旁组织中均表达,诱骗受体DcR1的mRNA在子宫内膜各组织中的表达率不同,但差异无统计学意义(P>0.05),而诱骗受体DcR2的mRNA在子宫内膜癌组织中的表达率明显低于正常子宫内膜组织和癌旁组织,差异具有统计学意义(P<0.005),癌旁组织细胞中DcR2表达和正常子宫内膜组织相近(P>0.05)。结论:TRAIL诱骗受体DcR1、DcR2在子宫内膜癌中的低表达可能与其发病机制有关,可能对防止子宫内膜癌变具有一定作用。
Objective：To investigate the expression of death receptors（ DR4, DR5 ） and decoy receptors（ DcR1, DcR2） of TNF-related apoptosis inducing ligand（TRAIL） in normal endometrium tissues,carcinoma endometrium tissues and the adjacent tissues. Methods： Reverse transcriptase polymerase chain reaction（RT-PCR） was used to detect mRNA positive expressions of the receptors of TRAIL in 20 cases of normal endometrium tissues,20 cases of carcinoma endometrium tissues and the adjacent tissues. Results ： DR4 and DR5 were expressed in normal endometrium tissues, carcinoma endometrium tissues and the adjacent tissues. DcR1 expressed differently in the tissues of endometrium, but with no statistical significance （ P 〉 0.05 ） ;the expression of DcR2 was much lower in the carcinoma endometrium tissues than in normal or tumor adjacent tissues （ P 〈 0. 005 ） ; the expression of DcR2 was similar in normal and tumor adjacent tissues（ P 〉 0.05 ）. Conclusions： The low expression of DcR2 in the carcinoma of endometrium may be concerned with its pathogenesis,which might play a role in preventing endometrium from carcinomatous change.
出处
《蚌埠医学院学报》
CAS
2008年第1期12-14,共3页
Journal of Bengbu Medical College
基金
安徽省教育厅自然科学研究资助项目(2003kj69)
