摘要
根据已知的日本血吸虫菲律宾株的副肌球蛋白分子的部分ｃＤＮＡ序列，设计两对寡核苷酸引物（引物１／２和引物３／４），以聚合酶链式反应（ＰＣＲ），用引物１／２从本室两个日本血吸虫中国大陆株ｃＤＮＡ库中均扩增出与预期大小（９２７ｂｐ）一致的特定ＤＮＡ片段。巢式ＰＣＲ——以第一扩增产物为模板，用引物３／４扩增出约５００ｂｐ的单一条带，与预期片段（４９４ｂｐ）大小一致。表明ＰＣＲ产物为编码副肌球蛋白的目的基因片段。
Two pairs of PCR primers(primers1 and 2; primers 3 and 4) were designed and synthesized according to the cDNA sequ enceencoding about half of the paramyosin molecule of S. japonicum (Philippines strain). When primers 1 and 2 were used for PCR, a specific DNA fragment was amplified from two S. japonicum (Chines eMainland Strain) adult cDNA libraries constructed in bacteriophage λgtll , which was accordant with the expected fragment of 927 bp. By use of the first PCR product acting as templet, a fragment, about 500 bp long, was amplified by nest PCR with primers 3 and 4, which was also in agreement with the expected length of 494 bp. Therefore, it is quit eevident that the first PCR product is the specific DNA fragment coding for paramyosin of S. japonicum (Chinese Mainland Strain).
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
1997年第2期128-131,共4页
Journal of Nanjing Medical University(Natural Sciences)
