摘要
从江苏和海南甜瓜种植区的病株中分离得到4种甜瓜蔓枯病病原物，在25℃、16h光照／8h黑暗条件下，4种病原菌分离物在PSA上培养后进行形态观察。结果表明：4种病原菌在PSA培养基上菌落形状相似，初为圆形后转变为同心圆；气生菌丝起初均为乳白色，后期为青灰色或者黑色，气生菌丝生长旺盛；其中C的基内菌丝较其它3种发达，可以直接产生大量的分生孢子，其余3种只能形成极少分生孢子；4种病原菌产生的孢子大小、形态基本一致，无色无隔或单隔为主；4种病原菌的菌丝生长速度差异不显著；在12h紫外灯／12h黑暗周期下处理4—6d，4种病原菌都能产生大量的分生孢子。此外，以感病品种白皮脆为供试材料，利用孢子悬浮液喷雾接种方法对这4种病原菌进行致病力的测定。结果表明，4种病原菌均具有较强的致病力，但它们之间的致病力差异不显著。
4 Kinds of diseases caused by Didymella bryoniae sepatated from melon cultivated area in Jiangsu and Hainan under 25℃ of 16/8 h light condition were observed. The analysis results indicated that the bacteria colony on PSA medium are similar to one another by their appearance. The 4 kinds of agent change into circular, then concentric circle; aerial mycelium first appears laete, then changes into cinereous or black, aerial myeelium grows luxuriantly. Among them, C hypha is more developing, compared with others, which can produce a great deal of branch spore directly. The other 3 kinds of agent can become a few branch spores. The size and appearance of the other 4 kinds of agent are basically consistent with one another, living together or living separately without any colour. The difference of growth coefficient of hypha of 4 kinds of agent is not significant; The 4 kinds of agent can produce a lot of branch spores under utraviolet light 12 h/dark period 12 h with the treatment of 4-6 days.In addition, taking white skin frailty as the tested material, the determination of the effect of the spore suspended liquid spraying on pathogenic rate of the 4 kinds of agent is conducted. The result indicated that the 4 kinds of agent have strong pathogenic effect, but among them, there is no sigenificant difference.
出处
《新疆农业科学》
CAS
CSCD
2008年第1期28-30,共3页
Xinjiang Agricultural Sciences
基金
新疆维吾尔自治区高技术研究与发展计划项目(200511106)
关键词
甜瓜
蔓枯病
形态特征
致病力
melon
Didymella bryoniae
appearance characteristic
pathogenicity
