摘要
以天津短杆菌（BrevibactetiumTianjin）T6-13为出发菌株，经亚硝基胍诱变处理，通过3种不同培养基的两组组合对接模式，直接快速地筛选出异柠檬酸裂解酶（ICL，E．C．4．1．3．1）缺失（icl-）的突变型菌株Tbm－30该突变型菌株的产酸水平和转化率比出发菌株提高29％左右，是一株以生化代谢理论设计筛选的具特征生化遗传标记的谷氨酸生产菌株，大大提高了筛选育种效率。
The mutant Tbm-3with icl- (the isocitrate lyase deficieney) biochemical genetic marker was obtained directly from the parent strain T6-13, (Brevibacterium Tianjin) by selective breeding according to the quick--screen type (inoculated separately on two groups of three kinds planting culture medium) after mutagenesis using N-nitro-N'-methyl--N--nitroso--guanidine The L-Glu level accumulated and yield of L--Glu using Tbm--3was raise about 29 % separately against that using T6-13. This is a L-Glu--producing mutant with specific biochemical genetic markerby selective breeding using the quick--screen type that was designed according to metabolism theory of biochemistry. This method raised the efficiency of selective breeding greatly.
出处
《食品科学》
EI
CAS
CSCD
北大核心
1997年第8期7-10,共4页
Food Science
关键词
快速筛选模式
生化缺陷型
菌株
谷氨酸
Quick-screen type
Mutation breeding
Isocitrate lyase
Biochernical defecitive mutant
