摘要
目的建立神经胶质瘤双向电泳技术。方法利用不同体积的裂解液提取大鼠脑组织中的蛋白质,并通过不同的蛋白质上样量(1、2、3mg),进行双向电泳,考马斯亮蓝染色,图谱分析。结果在等电点3～10,相对分子质量6.5～200KDa范围内分离得到蛋白质斑点为,1mg蛋白质上样量为574个蛋白质斑点,2mg为798个,3mg为803个斑点。2mg蛋白质上样量的双向电泳图谱更清晰,分离更好。结论成功建立了神经胶质瘤蛋白质的双向电泳技术。
Objective To establish a two-dimensional electrophoresis technology on brain tissue protein of rat. Methods Lysis buffer of different volume was taken to extract brain tissue proteins of rat, and different protein quantities（ 1 mg,2 mg,3 mg）were taken to establish a two-dimensional electrophoresis. Coomassie bril- liant blue was applied to stain protein, and patterns were analyzed. Results With a molecular mass between 6.5 - 200 KDa and isoelectric points（pI） from 3 - 10,1 mg proteins obtained 574 protein spots,2 mg 798 ,and 3 mg 803. Conclusion A two-dimensional electrophoresis technology on brain tissue protein of rat has been established successfully.
出处
《中国实用医药》
2008年第6期6-8,共3页
China Practical Medicine
基金
四川省卫生厅科研课题(项目编号:060220)
关键词
蛋白质组学
双向电泳
脑组织
裂解液
Proteomics
Two-dimensional electrophoresis
Brain tissue
Lysis buffer
