摘要
目的：测定慢性肺心病患者红细胞膜流动性改变及血浆过氧化脂质（ＬＰＯ）和超氧化物歧化酶（ＳＯＤ）活力对红细胞膜流动性的影响。方法：对４５例肺心病急性发作期患者，按病情分为Ⅰ，Ⅱ两组，２１名健康人，用荧光探针ＤＰＨ（１，６－Ｄｉｐｈｅｎｙｌ－１，３，５－ｈｅｘａｔｒｉｅｎｅ）测定红细胞膜流动性。同时测定血浆ＬＰＯ、ＳＯＤ活性。结果：对照组肺心病Ⅰ、Ⅱ组红细胞膜荧光偏振度值分别为０．２１６２±０．０１９８、０．２４３１±０．０１４７、０．２５２６±０．０１３５（Ｐ＜０．０１）；ＬＰＯ分别为１．５９８５±０．４８２６、１．９３３３±０．４１９９、２．００２９±０．５３１９（Ｐ＜０．０５）；ＳＯＤ活性分别为４１．６５０±１１．７４０、３５．１００±１０．６０、２６．４０４±８．２１５（Ｐ＜０．０１）；相关分析显示膜荧光偏振度与ＬＰＯ呈正相关（ｒ＝０．４１９６，Ｐ＜０．０５）与ＳＯＤ活性呈负相关（ｒ＝－０．４２８３，Ｐ＜０．０５）。结论：肺心病急性发作期患者红细胞膜流动性降低，对疾病的加重有一定影响，ＬＰＯ增加和ＳＯＤ活性降低是影响红细胞膜流动性的重要因素之一。
Objective: To explore the effects of the activity of lipid peroxide and superoxidation dismutase on membrane fluidity of erythrocyte in patients with chronic cor pulmonale. Methods: The membrane fluidity of erythrocyte and the activity of lipid peroxide (LPO) and superoxidation dismutase (SOD) were measured in 45 patients with chronic cor pulmonale (divided into two groups, i.e. CP Ⅰ,CP Ⅱ, according to patient′s condition) and 22 healthy individuals. Results: The membrane fluorescence polarization degree of erythrocyte was significantly increased and the membrane fluidity of erythrocyte remarkably decreased (P<0.001 and P<0.001). In the plasma of the patients, the activity of LPO was significantly increased (P<0.05) while that of SOD markedly decreased (P<0.05). Linear regression analysis showed that fluorescence polarization degree of the erythrocyte membrane was positively correlated with LPO (r=0.4196, P<0.05) but negatively correlated with SOD (r=-0.4283, P<0.05) in these patients. Conclusion: The membrane fluidity of erythrocyte is decreased in patients with chronic cor pulmonale. The increase of LPO activity and decrease of SOD activity are one of the important factors affecting membrane fluidity of erythrocyte.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1997年第4期366-368,共3页
Journal of Third Military Medical University
关键词
肺心病
膜流动性
过氧化脂质
超氧化物歧化酶
cor pulmonale
erythrocyte
membrane fluidity
lipid peroxide
superoxidation dismutase
