摘要
目的用基因芯片技术研究电磁脉冲(EMP)对小鼠肠组织的生物效应。方法将12只小鼠随机分为正常组和EMP组(每组6只),用200kV/m,200次的电磁脉冲辐照大鼠,于照后第18h,活杀小鼠,取空肠,提取RNA,经反转录后用Cy3、Cy5荧光标记,获得两组动物来源cDNA探针;cDNA探针与基因表达谱芯片进行杂交,结果由扫描仪扫描并用软件进行分析统计。结果EMP组与正常组相比,有56条基因表达发生了明显变化,其中有37条基因表达水平明显升高,而19条基因表达量明显下降。在表达异常的基因中有19条基因是已知功能或部分功能的基因,其中α-catenin胞质蛋白、淋巴细胞同型抗原、谷氨酰果糖6磷酸氨基转移酶、核糖体蛋白S17、小富脯氨酸蛋白2A、腺状激肽释放酶、脂氧合酶、醛酮还原酶、RNA结合蛋白、α-胰淀粉酶2、弹性蛋白酶2、p6-5淋巴细胞抑制因子基因等12条为表达上调基因;Jam新连接粘附分子、精氨酸甲基转移酶,Carm1、NNP-1、2-5寡腺苷酸合成酶、Mlark、ATP合成酶α亚基、解偶联蛋白基因等7条为表达下调基因,其余37条则为未知功能的基因。结论电磁脉冲辐照可诱导小鼠肠组织多个基因特异性表达,且上调基因数居多。
Objective To explore the bio-effects of electromagnetic pulse（EMP） on mouse small intestines induced by means of gene chip. Methods Twelve BALB/c mice were randomly assigned to the normal control group and the EMP group with 6 in each group. The EMP group was irradiated with 200 kV/m, 200 pulses EMP. 18 hours after the irradiation, the mice were sacrificed and their jejunum of small intestines were eviscerated. The fluorescent cDNA probes labeled with Cy3 and Cy5 were prepared from RNA extracted from the intestines of the two groups. Probes of the two groups were then hybridized against cDNA gene chip, the fluorescent signals were scanned with a scanner and the results were analyzed by computer. Results Compared with the control, 56 genes in gene expression profile were altered. The expression levels of 37 genes were up-regulated distinctly while 19 genes were down-regnlated significantly. Among the 56 genes, 19 were reported with known or inferred functions, 12 up-regulated genes were catenin alpha 1 （α-catenin）, ly-6 alloantigen（ Ly-6E ）, fructose-6-phosphate transaminase （GF6P）, ribosomal protein S17 （ rpS17 ）, small proline-rich protein 2A （Sprr2a）, glandular kallikrein27 （GK27）, lipoxygenase-3, aldo-keto reductase （Akrlc12）, GSG1, amylase 2 （Amy2） ,elastase 2, p6-5 gene and 7 down-regulated genes were junctional adhesion molecule （Jam）, protein arginine methyltransferase （Carrel） ,NNP-1, 2-5 A synthetase L2,Mlark gene, ATP synthase alpha subunit, uncoupling protein-2 （ Ucp2） gene; the other 37 were reported with unknown functions. Conclusion EMP irradiation could induce specific expressions of some genes in mouse small intestines and most of these genes were up-regulated ones.
出处
《卫生研究》
CAS
CSCD
北大核心
2008年第1期22-24,共3页
Journal of Hygiene Research
基金
国家自然科学基金资助(No30370440,30570447)
关键词
电磁脉冲
基因芯片
肠组织
小鼠
electromagnetic pulse （EMP）, gene chip, small intestine, mouse
