摘要
检测白血病或肿瘤细胞多药耐药基因(mdr 1)有多种方法,其中以RT-PCR法相对更灵敏、简单和特异.目前国内多用β_2微球蛋白(β_2MG)为内参照,以mdrl/β_2MG比值进行相对定量.现已发现β_2MG可受肿瘤影响.本文以细胞高表达的组织蛋白H 3.3(H 3.3)为内参照,建立了RT-PCR mdrl基因表达方法,并对临床10例标本进行检测.本文探讨了内参照应用的价值、存在的问题以及临床应用的意义.
Many different methods have been used to evaluate mdrl expression in leukemiasand cancer cells. RT-PCR is recommended because of its relative sentitive, simplicity and specitici-ty. At least, some other groups have quantified mdrl by β2MG as an internal standard, then got arelative quantitation of mdrl by mdrl/β2MG. But β2MG has been affected by some malignancies.Here we developed a assay that measures mdrl expression using Histone 3. 3 as control gene byRT-PCR. We have used this assay to measure mdrl expression in 10 clinical specimens. This pa-per discussed some problems of the control gene in clinical application and its significance.
出处
《海军总医院学报》
1997年第1期9-13,共5页
Journal of Naval General Hospital of PLA
