摘要
目的:建立力达霉素辅基蛋白荧光衍生化的方法。方法:采用FITC和9-氨基芴分别对力达霉素辅基蛋白的氨基端和羧基端进行衍生化。偶联产物用Sephadex G15葡聚糖凝胶柱和半透膜分离纯化,并用MALDI-TOF质谱检测连接效果。结果:在本试验条件下,力达霉素辅基蛋白分别与FITC和9-氨基芴成功偶联,偶联连接分子比均为1:1。结论:此法简单和准确,可用于制备力达霉素辅基蛋白荧光衍生物。
Objective： To set up methods of fluorescence derivatization of lidamycin apoprotein. Method ： Amino groups and carboxyl groups of lidamycin apoprotein were derivatized with FITC and 9-aminoflurene respectively. The conjugates were separated with Sephadex G15 gel column and were pured with semipermeable membrane. The conjugates were identified by MALDI-TOF. Result： Under this condition, lidamycin apoprotein was conjugated successfully with FITC and with 9-aminoflurene respectively, In both of the conjugates, the ratio of the two molecules is 1： 1. Conclusion： The method is simple and accurate, can be applicated for synthesize th fluorescence derivatization of lidamycin apoprotein.
出处
《中国药师》
CAS
2008年第1期16-18,共3页
China Pharmacist
