摘要
目的:针对羧甲基壳聚糖(CMC)作为防止术后粘连材料的应用,研究不同取代和分子量的CMC的抑菌活性。方法:通过测定细菌培养液的浊度(OD值)来考察CMC对大肠埃希菌和铜绿假单胞菌(G^-)以及金黄色葡萄球(G^+)的抑菌活性。结果:CMC的抑菌性能与其分子量、羧甲基的取代度与取代位置、细菌的种类和浓度有关;N,O-CMC抑菌作用比N-CMC或O-CMC强,对金黄色葡萄球菌和大肠埃希菌的抑菌活性随着羧甲基取代度的增加而增强;分子量低的N,O-CMC对金黄色葡萄球菌和铜绿假单胞菌的抑制作用强于高分子量的N,O-CMC;CMC对金黄色葡萄球菌的抑菌作用最好,对大肠埃希菌次之,对铜绿假单胞菌的抑制作用较弱。在一定浓度范围内,CMC的浓度越高,抑菌效果越好。而且溶剂类型影响壳聚糖的抗菌活性。结论:CMC具有广谱的抑菌活性。
Objective： The antibacterial activity of CMC was investigated as a postoperative adhesion preventive, Method： Antibacterial activities against gram-negative （Escherichia coli, E. coli and Pseudomonas aeruginosa） and gram-positive bacteria （Staphylococcus aureus） were explored by the optical density method. Result： Antibacterial activities of CMC was related to its molecular weight, positions of substituents, degree of substitution, species of bacteria and concentration in solution. N, O-CMC generally showed remarkably stronger bactericidal effects than N-CMC and O-CMC. Antimicrobial capacity of N, O-CMC against S. aureus was increased as degree of substitution was increased. The antibacterial activity of N, O-CMC with higher degree of substitution against E. coli were more effective than that of N, O-CMC with lower degree of substitution. The antimicrobial test demonstrated that antimierobial capacity of N, O-CMC was influenced by its molecular weight slightly in the presence of 0.1% CMC, lower molecular weight resulted in better antimicrobial ability against P. aeruginosa and S. aureus. The antibacterial activities of N, O-CMC was also related to the species of bacteria. CMC showed stronger bactericidal effects with gram-positive bacteria than gramnegative bacteria. CMC with higher molecular weight were ineffective in inhibiting the growth of P. aeruginosa. It was also found that the antibacterial activities of N, O-CMC-2 was increased as its concentration was increased. Bactericidal effects of CMC were affected by solvent. Conclusion： CMC have antibacterial activities of gram-negative and gram-positive bacteria.
出处
《中国药师》
CAS
2008年第1期9-12,共4页
China Pharmacist
基金
广东省科技攻关项目(2KB02902G)
深圳市科技基金(No.JH20050300531A)
关键词
羧甲基壳聚糖
抑菌作用
取代度
分子量
Carboxymethyl chitosan
Antimicrobial activities
Molecular weights
Degree of substitution
