摘要
在小麦麸皮中阿拉伯木聚糖酶解工艺初步研究的基础上,采用二次回归正交旋转组合设计对其酶解工艺进行了优化。建立了水溶性阿拉伯木聚糖(WEAX)得率(Y)对温度(X1)、时间(X2)、酶用量(酶与底物质量比,g/g)(X3)及pH值(X4)四个试验因素的正交回归模型:Y=12.84921-1.36983X1+0.69076X2+0.75443X3-1.46276X12-0.93773X42。从模型推知,当温度为60℃、时间为3h、酶用量为0.75%、pH值为5时,得率最大值达到15.28%,验证实验结果与模型值相符。
Based on the primary studies on the enzymatic hydrolysis conditions of arabinoxylan in wheat bran, the enzymatic degradation conditions of arabinoxylan were optimized by quadratic regression orthogonal rotary tests. The quadratic orthogonal regression model is of arabinoxylan yield （Y） to four factors of temperature （X1）, time （X2）, the concentration of enzyme （X3） and pH value （X4） and established as Y=12.84921 - 1.36983X1＋0.69076X2＋0.75443X3- 1.46276X1^2 - 0.93773X4^2. It was concluded from the model that when temperature, time, enzyme concentration and pH value are 60 ℃, 3 h, 0.75% and 5 respectively, the yield （Y） is the maximal （15.28%） and consistent with the confirmatory experiment result.
出处
《食品科学》
CAS
CSCD
北大核心
2008年第1期141-145,共5页
Food Science
基金
中国农业科学院科研基金项目
关键词
阿拉伯木聚糖
戊聚糖酶
酶解工艺
正交旋转组合设计
优化
arabinoxylan
pentosanase
enzymatic hydrolysis conditions
orthogonal rotation combination designoptimization
