摘要
目的研究芳香烃受体（AhR）基因G1661A多态性与焦炉工尿中1-羟基芘水平的关系。方法选取214名男性焦炉工和81名无多环芳烃职业性接触的男性工人为研究对象，收集研究对象的年龄、吸烟和饮酒、职业暴露史等信息，应用等位基因特异性扩增（ASA）方法检测AhR基因型，使用高效液相色谱-荧光检测器法测定工人尿中1-羟基芘的水平。结果G／G、G／A和A/A3种基因型在接触组中的分布频率分别为52．8％（113／214）、27．6％（59/214）和19．6％（42/214），在对照组的分布频率分别为67．9％（55/81）、19．8％（16／81）和12.3％（10／81），2组基因型分布频率比较，差异无统计学意义（P〉0．05）；接触组G、A等位基因频率分别为66．6％（285/428）和33．4％（143/28），对照组分别为77．8％（126／162）和22．2％（36／162），2组等位基因频率比较，差异无统计学意义（P〉0．05）。经过广义线性模型对工人的作业工龄和外暴露等级校正后，用协方差分析方法分析发现，A/A、G／A、G／G3种基因型者对数转换后尿中1-羟基芘的水平分别为（3．62±0．12）、（3．43±0．12）和（3．44±0．08）μmol/mol Cr，携带G、A等位基因的个体对数转换后尿中1-羟基芘的水平分别为（3．24±0．09）和（3．43±0．10）μmol/molCr，不同基因型及等位基因型的焦炉工尿中1-羟基比水平比较，差异无统计学意义（P〉0．05）。结论焦炉工AhR基因G1661A多态性对尿中1-羟基芘的水平无明显影响。
Objective To study the relationship between polymorphism of aryl hydrocarbon receptor （AhR） gene in G1661A and the level of urinary 1-hydroxypyrene among coke oven workers. Methods 295 male subjects were studied,including 214 workers working in coke oven plant and 81 controls working in raw material plant who were not generally exposed to polycyclic aromatic hydrocarbons occupationally. General information of subjects were collected in a specific questionnaire including age, smoking and drinking habits, the history of occupation and so on. The AhR genotypes were detected by allele specific amplification（ASA）,and the levels of urinary 1-hydroxypyrene were measured by high performance liquid chromatography with a fluorescence detector. Results The frequencies of G/G, G/A and A/A genotype were 52.8% （1 13/214）,27.6% （59/214） and 19.6% （42/214） in exposed group and 67.9% （55/81）, 19.8% （16/81） and 12.3% （10/81） in con-tml group,respectively. No significant difference was found in three genotypes between the exposed and control group. Allele frequencies of G and A were 66.6% （285/428） and 33.4% （143/428） in exposed group and 77.8%（126/162） and 22.2%（36/162） in control group,and no statistical differences were found in allele frequency between exposed and control group. After the length of service and external exposure orders in general linear model were adjusted, results of covariance analysis showed that logarithmic transformed urinary 1-hy- drox-ypyrene levels were （3.62±0.12）, （3.43±0.12） and （3.44±0.08） μmol/mol Cr in individuals with A/A,G/ A and G/G, respectively. The logarithmic transformed urinary 1-hydroxypyrene levels were （3.24±0.09） and （3.43±0.10） μmol/mol Cr in individuals with allele of G and A. No statistical differences were found in level of 1-hydroxypyrene among A/A, G/A and G/G genotype individuals, and between allele G and allele A after external exposure orders and length of service were adjusted. Conclusion The polymorphism of argl hydrocarbon receptor G1661A has no significant impact on levels of urinary 1-hydroxypyrene.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2008年第1期20-23,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金项目（30471437）
关键词
烃类
芳香
多态性
单核苷酸
芘类
Hydrocarbons, aromatic
Polymorphism, single nucleotide
Pyrenes
