摘要
目的:研究壳聚糖/igf-1基因(CS/igf-1)复合物对体外培养的骨髓基质细胞(MSC)分化及增殖的影响。方法:自大鼠股骨及胫骨组织中分离培养大鼠骨髓基质细胞,以含有人igf-1基因cDNA的哺乳动物表达载体pTRacer-igf-1与壳聚糖制备成CS/igf-1复合物加入细胞培养基中进行干预;另设空白细胞组、CS/空质粒干预组及单纯CS干预组作为对照。3H-TdR掺入法、甲苯胺兰染色分别检测细胞的增殖情况及成软骨活性。结果:与空白对照细胞相比,壳聚糖可使所培养的骨髓基质细胞表现出明显的成软骨活性。igf-1基因的参与则可显著促进细胞的增殖。结论:CS/igf-1复合物可促进对体外培养的骨髓基质细胞增殖并向成软骨细胞分化。
Obiective： To study the effect of chitosan or chitosan/igf-1 （CS/igf-1） gene complex on the proliferation and differentiation of bone marrow stroma cell（MSC）. Methods： Rat MSC separated from bone marrow was primarily cultured in DMEM medium with low glucose concentration. The cells were separated into four groups and treated with chitosan, CS/igf-1 or CS/plasmid correspondingly. At the end of culture, cell proliferation was detected by ^3H-TdR incorporation while Cartilage-genesis was determined by methylbenzene amino blue staining. Results： Compared with normal control group, there was significant increase of chondro-genesis in CS/igf-1 treated cells. Cartilage-genesis was significantly stimulated with igf-1 gene. Conclusion： The results suggested that CS/igf-1 gene complex can stimulate cell proliferation and enhance chondro-genesis from MSC.
出处
《天津医药》
CAS
北大核心
2008年第1期35-37,共3页
Tianjin Medical Journal
基金
天津市自然科学基金重点项目(项目编号:06YFJZJC00800)
天津市高等学校科技发展基金项目(项目编号:20040104)
关键词
骨髓细胞
壳聚糖
细胞分化
细胞增殖
软骨细胞
bone marrow cells chitosan cell differentiation cell proliferation chondrocytes
