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线粒体基因突变型糖尿病患者氧化应激状态的临床研究 被引量：1

Clinical Study on Oxidative Stress in Patients with Mitochondrial Diabetes Mellitus
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摘要 目的:观察线粒体基因突变型糖尿病(mitochodrial diabetes mellitus,mtDM)患者体内氧化应激状态,探讨线粒体基因突变与氧化应激的关系。方法:应用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)筛查2型糖尿病患者(T2DM组)和健康对照组(C组)mtDNA tRNALE(UUUR)3243A→G(A3243G)和mtDNAND412026A→G(A12026G)突变位点发生情况,用酶反应化学比色法测定线粒体基因突变型糖尿病组(mtDM组)、T2DM组及健康对照组外周血中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、抗活性氧活力(Anti-ROS)和丙二醛(MDA)等氧化应激指标。结果:(1)与C组相比,mtDM组和T2DM组Anti-ROS、SOD、GSH-Px、CAT活性及SOD/MDA均下降(P<0.05),MDA含量升高(P<0.05或P<0.01)。(2)mtDM组与T2DM组相比,CAT活性下降(P<0.05),MDA含量升高(P<0.05或P<0.01)。(3)A3243G突变组和A12026G突变组之间SOD、GSH-Px、CAT、Anti-ROS活性和MDA差异无统计学意义(P>0.05)。结论:线粒体基因突变型糖尿病较无突变2型糖尿病患者及健康对照组氧化应激损伤更严重,可能与线粒体基因突变有关。 Objective： To investigate the oxidative stress （OS） in patients with mitochondrial diabetes mellitus and analyze the relationship between mitochondrial DNA （mtDNA） mutation and OS. Methods： Firstly,A3243G and A12026G mutations were detected by PCR-RFLP in patients with type 2 diabetes mellitus（T2DM） group and control（C） group. Secondly, oxidative stress indexes of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, catalase （CAT）, anti-reactive oxygen species （Anti-ROS） and malondialdehyde （MDA） were measured by colorimeter. Results： （1）Compared with that in control group,activities of anti-ROS,SOD,GSH-Px,CAT and SOD/MDA were decreased and MDA were increased in both mtDM and T2DM groups （P 〈 0.05 or P 〈 0.01 ）, respectively. （2）The mtDM group had more decreased activities of CAT and increased MDA compared with the T2DM group （P 〈 0.05 or P 〈 0.01 ）. Conclusion： There is more serious oxidative injury in the mtDMA group, which may due to the mtDNA mutation.
作者 郑辉 于德民 李明珍 于珮 刘德敏
机构地区 天津医科大学代谢病医院
出处 《天津医药》 CAS 北大核心 2008年第1期7-10,共4页 Tianjin Medical Journal
基金 天津市高等学校科技发展基金资助项目(项目编号:20030326)
关键词 糖尿病 2型 突变 DNA 线粒体 氧化性应激 过氧化氢酶 丙二醛 diabetes mellitus,type 2 mutation DNA, mitochondrial oxidative stress catalase malondialdehyde
分类号 R339.38 [医药卫生—人体生理学] R114 [医药卫生—卫生毒理学]
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参考文献7

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二级参考文献9

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共引文献2

同被引文献4

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天津医药
2008年 第1期

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