摘要
目的:研究原发性肝癌中DNMT3b基因剪接变异体的表达情况及其在肝癌发生发展中的作用。方法:通过RT-PCR的方法对30例肝癌、30例癌旁组织中DNMT3b基因的剪接变异体进行检测,并将结果与肝癌患者临床指标进行统计学分析。结果:RT-PCR结果显示DNMT3b基因以四种剪接变异体(DNMT3b1、DNMT3b3、DNMT3b4和DNMT3b5)的形式表达,其总的表达率肝癌组为93.3%(28/30),癌旁组为93.3%(28/30)。其中DNMT3b4在肝癌组中的表达高于癌旁组(P<0.05),与肝癌病理分化和CLIP评分无相关性(P>0.05)。结论:DNMT3b基因剪接变异体DNMT3b4可能参与肝癌的发生演变。
Objective： To study the splice variants of DNMT3b gene in primary hepatocellular carcinoma, and their effects on the development of hepatocellular carcinoma. Methods： Thirty hepatocellular carcinoma （HCC） tissues and their corresponding adjacent para-cancerous tissues were examined. RT-PCR was used to detect the splice variants of DNMT3b gene. The relationship between the laboratory results and clinicopathological characteristics of HCC was analyzed as well. Results： The expressions of DNMT3b gene were detected by RT-PCR in the forms of alternative spliced variants of DN- MT3b1, DNMT3b3, DNMT3b4 and DNMT3b5. Their total expressions were 93.3% （28/30） in HCC and 93.3% （28/30）in para-cancerous tissues. There was a higher DNMT3b4 expression in HCC than that in para-cancerous tissues （P〈0.05）. There was no relationship between pathological differentiation and CLIP grade （P〉0.05）. Conclusion： DNMT3b4, one of the alternative spliced forms of DNMT3b, may play an important role in hepatic carcinogenesis.
出处
《天津医药》
CAS
北大核心
2008年第1期1-3,共3页
Tianjin Medical Journal
基金
天津市科委科技攻关项目(项目编号:05YFSJSF02500)
天津市科委科技创新能力与环境建设平台项目(项目编号:05SYSYJC02600)
关键词
肝肿瘤
癌
剪接体
立体异构现象
基因表达
liver neoplasms carcinoma spliceosomes stereoisomerism gene expression
