摘要
目的探讨微波辐射后大鼠海马线粒体呼吸链相关基因细胞色素C氧化酶（COX）表达的变化。方法采用30mW／cm^2微波辐射30只雄性Wistar大鼠，于辐射后6h、1d、3d和7d活杀取海马，采用逆转录-聚合酶链反应（reverse transcription—polymerase chain reaction，RT—PCR）和图像分析检测大鼠海马组织COX亚基Ⅰ、Ⅱ和ⅣmRNA表达变化，通过Western Blot和图像分析检测大鼠海马组织COXⅠ蛋白表达变化。结果辐射后大鼠海马COXⅠ与ⅡmRNA变化规律相似，即与假辐射组相比，于辐射后6h减少，1d增加，3d和7d见恢复；COXⅣmRNA于辐射后1d表达增加，3d和7d见恢复；大鼠海马COXⅠ蛋白于辐射后6h略降低，1d显著降低（P〈0．05），3d见增加，7d基本恢复。结论微波辐射后大鼠海马线粒体呼吸链相关基因COX表达降低，可能参与其能量代谢障碍的病理生理过程。
Objective To study gene expression of cytochrome c oxidase （COX） subunit in hippocampus of rats after microwave exposure. Methods Thirty male Wistar rats were exposed to microwave radiation at 30 mW/cm^2, Rats were sacrificed at 6 h, 1 d, 3 d and 7 d after exposure. Reverse transcription-polymerase chain reaction （RT-PCR） and image analysis were used to study the expression of COX Ⅰ, Ⅱ and Ⅳ mRNA. The expression of COX Ⅰ protein was detected by Western Blot and image analysis. Results The changes of COX Ⅰ and ⅡmRNA had the similar regular pattern which were decreased at 6 h after exposure and increased at 1d, while retrieved 3 d after. The expression of COX Ⅳ mRNA was increased at 1 d, while retrieved 3 d and 7 d after. The expression of COX Ⅰ protein was a little decreased at 6 h, and decreased at I d significantly （ P 〈 0.05 ） , while increased at 3 d and retrieved at 7 d. Conclusion It manifested that COX Ⅰ, II and Ⅳ of the mitochondria influenced energy production and participated in the process of dysbolism.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2008年第1期31-33,共3页
Chinese Journal of Physical Medicine and Rehabilitation
基金
军队医药卫生“十一五”专项基金（062065）
