摘要
采用常规的细菌分离纯化方法从土壤中分离出絮凝剂产生菌菌株,经过驯化培养后,以发酵液对高岭土混悬液的絮凝效果为指标,筛选出1株高效絮凝剂产生菌。采用单因素试验方法和正交试验设计方法,分析了影响絮凝效果的主要因素,对菌株的最佳培养条件进行了优化研究。结果表明:菌株S3产絮凝剂的最佳培养条件是碳源为葡萄糖(20 g/L),氮源为酵母膏(2.5 g/L),培养温度为28℃,初始pH值为8,通气量为50 r/min;在此条件下,絮凝率为92%左右。
In this study, strains of bioflocculant-producing microorganisms were screened from soil sample by conventional methods. 1 strain with high flocculating activity was obtained after rescreening with kaolin clay suspension flocculation. Then the effect of the cultural conditions and the medium composition and proportion on bioflocculant production was studied by single factor test and orthogonal design test. The experimental results showed that the medium composition and proportion and other cultural conditions had significant effect on bioflocculant production. The optimum culture conditions of strain S3 were as follows, carbon source was glucose （20 g/L）, nitrogen source was yeast extract （2.5 g/L）, cultural temperature 28℃, initial pH 7, rotating speed 50 r/min. Under this condition, the flocculating rate was about 92%.
出处
《西北农业学报》
CAS
CSCD
北大核心
2008年第1期120-123,共4页
Acta Agriculturae Boreali-occidentalis Sinica
关键词
微生物絮凝剂
筛选
培养条件
正交设计
Bioflocculant
Screening
Cultural conditions
Orthogonal design
