摘要
目的:研究白藜芦醇对喉癌细胞系Hep-2细胞生长的抑制作用。方法:在显微镜下计算细胞密度,MTT法绘制细胞生长曲线并计算细胞生长抑制率,利用软琼脂集落形成实验在倒置纤维镜下观察白藜芦醇处理Hep-2细胞前后细胞形态学变化及计算集落形成率。结果:各浓度的白藜芦醇均可使细胞生长曲线降低,细胞倍增时间延长。白藜芦醇也可以明显抑制Hep-2细胞形成集落,并发现白藜芦醇对细胞形态的影响与药物的剂量有明显的关系。结论:白藜芦醇对喉癌细胞系Hep-2细胞生长的抑制具有时间和剂量依赖性。
Objective：To study the inhibitory effect of resveratrol on growth of human laryngeal cancer cell line, Hep-2. Method：Count cell number under microscope,MTT assay was used to determine the cell growth in- hibitory rate. Soft agar colony forming experiment was performed to observe the proliferation ability,before or after resverator treatment. Result：Resverator was able to depress cell growth and inhibit cell proliferation. Conclusion： Resveratrol strongly inhibit Hep-2 cell proliferation in a time- and dose-dependent manner.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2007年第24期1129-1131,共3页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
白藜芦醇抑制喉癌细胞生长的实验研究黑龙江省自然科学基金资助项目(No:D2004-15)
