摘要
沙眼衣原体所致泌尿生殖道感染是最常见的性传播疾病之一。对１１０例患者进行聚合酶链反应（ＰＣＲ）与直接免疫荧光法（ＤＦＡ）检测，比较二者在临床检测中的优缺点。ＰＣＲ引物为主要外膜蛋白基因片段，阳性６５例（５９１％），阴性４５例（４０９％）。ＤＦＡ阳性４３例（３９１％），阴性６７例（６０９％），ＰＣＲ和ＤＦＡ阳性率有显著性差异（Ｐ＜００１），病程长短对ＰＣＲ阳性率无明显影响（Ｐ＞００５），病程长短对ＤＦＡ阳性率有显著影响（Ｐ＜００１），病程长者ＤＦＡ阳性率低。ＰＣＲ特别适用于临床中病程长且ＤＦＡ阴性患者。
Chlamydia trachomatis infection is one of the prevalent sexaully transmitted diseases(STD) of the urogenital tract. A total of 110 clinical specimens from patients attending STD clinics were detected by using polymerase chain reaction (PCR), and were compared with direct fluorescent assay (DFA). Oligonucleotide primers were designed to amplify a fragment within the conserved region of a major outer membrane protein(MOMP) gene of Chlamydia trachomatis, the positive rate of PCR was 59 1% (65/110), and negative rate 40 9% (45/110). The positive rate of DFA was 39 1% (43/110), and negative rate were 60 9% (67/110). The Chlamydia trachomatis positive rates were significantly different between PCR and DFA (P<0 01). The relationship between the course of disease and the detection rates by PCR and DFA was analyzed. The positive rate of PCR was not related to the course of disease (P>0 05),but the positive rate of DFA was related to the course of the disease, the longer the course of disease(>2 months) the lower the positive rate of DFA (P<0 01). It is the authers′ opinion that PCR is useful for those patients with long course of disease and negative DFA result.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1997年第3期167-169,共3页
Chinese Journal of Dermatology
关键词
沙眼衣原体
聚合酶链反应
直接免疫荧光法
Chlamydia trachomatis Polymerase chain reaction Direct fluorescent assay
