摘要
本实验以镰形扇头蜱吸血前后雄蜱唾液腺的抑制消减杂交cDNA文库中的EST数据为基础,应用RACE方法从镰形扇头蜱体内克隆出一个IgG(IgG binding protein,IGBP)基因的全长序列,该基因全长683bp,编码178个氨基酸,预测分子量为19.5kDa,经同源性比较该基因与具尾扇头蜱的IGBP-MB基因序列的同源性高达92%。用RT-PCR方法分析了该基因表达的性别特异性、各个器官、不同发育阶段的表达情况,结果表明,该基因表达没有性别特异性;IGBP基因在唾液腺、壳这2个器官有所表达,但在肠中却没有表达;在蜱的各个发育阶段均有表达。
The study was based on the EST data of Suppressed Subtractive Hybridizaiton cDNA library of fed and unfed male Rhipicephalus haemaphysaloides. Firstly, we obtained the full-length sequence of IgG binding protein （IGBP） from rapid amplification of cDNA ends protocol. The gene had 683 bp encoding 178 amino acid residues with 19.5 kDa predicted molecular weight and showed a rather high homology （92% cDNA identities） with Rhipicephalus appendiculatus IGBP-MB. Secondly, the expression feature of male specific, in the different organs and phages, were analysised by the reverse transcription polymerase chain reaction method （RT-PCR）, the results suggested the expression of IGBP gene had no male specific characteristic, and IGBP gene was expressed in the salivary, shell, eggs, unfed larva, unfed nymphae and adult, but not expressed in the gut.
出处
《寄生虫与医学昆虫学报》
CAS
2007年第4期225-230,共6页
Acta Parasitologica et Medica Entomologica Sinica
基金
国家863计划资助项目(No.2006AA10A207)
