摘要
本研究建立了西方马脑炎病毒的RT-PCR检测方法,应用于实验感染西方马脑炎病毒的来亨鸡血液及蚊虫样本中的病毒核酸检测。结果从感染病毒的来亨鸡血液及蚊虫样本中均能扩增出与预期分子量一致的特异性条带,经序列测定证明,与西方马脑炎71V-1658(NC-003908)病毒株核酸序列有较高的同源性。表明所建立的RT-PCR检测方法能够快速敏感地检测出实验感染来亨鸡血液样本及蚊虫体内西方马脑炎病毒。
A pair of primers were synthesized, and related RT-PCR assay was established to detect the western equine encephalitis virus （WEEV） in experimentally infected mosquitoes and Leghorn chicken. The optimal condition of RT-PCR was obtained by a serial tests. The results showed that the nuclear acid sequence of the PCR product was similar with those of 71V-1658 strain （Accession no： NC _ 003908）. The lower limit of RT-PCR detection determined using WEEV RNA extracted from serial dilutions of virus in infected mouse brain tissues was approximately 10- 7 dilutions. The RT-PCR assay could be reliably used as an efficient and rapid protocol of detecting WEEV in experimentally infected mosquitoes and Leghorn chicken.
出处
《寄生虫与医学昆虫学报》
CAS
2007年第4期213-217,共5页
Acta Parasitologica et Medica Entomologica Sinica
基金
国家部科技攻关课题(2003BA712A09-02)
关键词
西方马脑炎病毒
RT-PCR
蚊虫
来亨鸡
Western equine encephalitis virus
RT-PCR
Mosquito
Leghorn chicken
