摘要
通过MTT比色法和Westernblot法检测NasSeV5O18·3H2O的抗肿瘤活性及作用机理．结果表明，Na5seV5O18·3H2O（0．625-20mg·L^-1）能抑制K562细胞增殖（P〈0．05）；药物处理K562细胞24h，可使胞浆细胞色素C，IκBα含量明显增多，核内NF-κB含量减少．结果提示Na5SeV5O36·3H2O能体外抑制K562细胞增殖，其机理可能与线粒体凋亡和NF-κB／IκBα信号转导有关．
Aimed to study the effect and mechanism of selenium compound Na5SeV5O18 · 3H2O, MTT colorimetric method and Western blot were used to test anti-proliferation activity and the content of cytochrome C,NF-κB and IκBα in K562 cells. The results show that Na5SeV5O18 · 3H2O 0. 625~20 mg · L^-1 could significantly inhibit proliferation of K562 cells in vitro in a time and dose-dependent manner, IC50 values were 14.41 and 3.45 mg · L^-1 after treated for 48 h and 72 h, respectively, and the intracellular content of cytochrome C and IeBa markedly increased and NF-κB decreased when treatment for 24 h. The results imply Na5SeV5O18 · 3 H2O has anti-tumor activity and the mechanism of which may relate to apoptosis of mitochondria and signal pathway of NF-κB/ IκBα.
出处
《河南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第1期96-98,共3页
Journal of Henan Normal University(Natural Science Edition)
基金
973计划前期研究专项(2006CB708506)
