摘要
目的：观察长时间给予μ阿片受体肽类激动剂DAGO及非肽类激动剂OMF对μ受体基因表达的影响，并比较这两者作用的差异性。方法：采用反转录-聚合酶链反应（RT－PCR）方法。结果：（1）DAGO使μ受体mRNA水平显著降低，作用持续48h；而OMF作用只持续24h；（2）DAGO在10－8mol·L-1时即可使μ受体的mRNA水平显著降低，而OMF在10－7mol·L-1时才有效。结论：长时间给予肽类及非肽类μ激动剂均可以显著抑制μ受体的基因表达；DAGO作用较强，时程较长。
Objective: To observe the effects of highly-selective peptide μ opioid receptor agonists DAGO and non-peptideμ agonist OMF on the transcription of μ opioid receptor in cultured cell line SHSY5Y after a long-termexposure to opioids, and the possible difference between the two agonists. Methods: Reverse Transcription-polmperase chain reaction(RT-PCR). Results: (1) The level of μreceptor mRNA decreased significantly 12~48 h after the administration of DAGO; The effect of OMF lasted only for 24 h. (2) DAGO produced asignificant decrease of μ-receptor mRNA at 10-8 mol.L-1; whereas OMF was effective at l0-7 mol.L-l.Conclusion: tong-term administration of both peptide and non-peptide opioid agonists significantly decreasedgene expression of μopioid receptor at transcripion level and the effect of peptide agonist DAGO seems to bestronger and lasting longer than that of non-peptide agonist OMF.
出处
《北京医科大学学报》
CSCD
1997年第4期308-310,共3页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金!39440004
关键词
受体
阿片样
μ激动剂
基因表达
Receptors, opioid, mu/agonists Gene expression RNA, messenger/metab DAGO OMF
