摘要
在体外培养的家兔气道平滑肌细胞（ＡＳＭＣ）上，观察肾上腺髓质素（ＡＭ）对内皮素（ＥＴ）促ＡＳＭＣ增殖的影响及丝裂素活化蛋白激酶（ＭＡＰＫ）活性的变化。以探讨ＡＭ对ＡＳＭＣ增殖的调控。结果显示１０－８ｍｏｌ／ＬＥＴ－１显著刺激ＡＳＭＣ３Ｈ－ＴｄＲ参入及ＭＡＰＫ激活（Ｐ＜０．０１）。ＡＭ（１３－５２）呈剂量依赖地抑制ＥＴ－１的上述作用（Ｐ＜０．０５，Ｐ＜０．０１）。单独应用ＡＭ（１３－５２）对ＡＳＭＣ３Ｈ－ＴｄＲ参入及ＭＡＰＫ活性无明显影响。表明ＡＭ（１３－５２）可抑制ＡＳＭＣ对ＥＴ－１的增殖反应，其机理可能涉及ＭＡＰＫ活性的抑制。
In order to investigate the role of adrenomedullin(AM) in regulating the airway smooth muscle cells(ASMC) proliferation, the effect of AM on the proliferative response of ASMC to ET 1 was observed and the change of mitogen activated protein kinase(MAPK) activity was examined in cultured rabbit ASMC. The results showed: (1) ET 1(10 -8 mol/L) markedly stimulated the Thymidine ( 3H TdR) incorporation (p<0 01) and MAPK activation (p<0 01) of ASMC. (2) AM(13 52) (10 -9 ～10 -7 mol/L)concentration dependently inhibited the above effect induced by ET 1(p<0 05 or p<0 01),while AM(13 52) alone can not directly inhibit the ASMC 3H TdR incorporation and MAPK activation. These data suggest that AM can inhibit the proliferative response of cultured ASMC to ET 1 and the mechanism may involve its inhibitory effect on MAPK activity
出处
《基础医学与临床》
CSCD
1997年第4期64-67,共4页
Basic and Clinical Medicine
关键词
肾上腺髓质素
内皮素
气道平滑肌
哮喘
细胞增殖
adrenomedullin endothelin airway smooth muscle cell proliferation mitogen activated protein kinase
