期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

CpG-ODN促进小鼠骨髓来源的树突状细胞成熟作用的实验研究 被引量：2

Study on stimulatory effect of CpG-ODN on maturation of murine bone marrow-derived dendritic cells
暂未订购
导出
摘要 目的改进目前体外扩增树突状细胞(dendritic cell,DC)的方法,探讨含CpG序列的寡脱氧核糖核苷酸(CpG Oligodeoxynucleotide,CpG-ODN)促进DC成熟的作用。方法用500u/ml重组小鼠粒细胞巨噬细胞集落刺激因子(rmGM-CSF)和200u/ml重组小鼠白细胞介素-4(rmIL-4)诱导小鼠骨髓细胞体外分化成DC,采用相差显微镜、扫描电镜、FACS检测细胞表面分子和混合淋巴细胞反应(MLR)等方法比较CpG-ODN、TNF-α等不同培养方法促进DC成熟的作用。结果培养10d,可获得大量典型的DC,经FACS检测细胞表面分子、MLR等证实,CpG-ODN具有较强的促进DC成熟的作用。结论CpG-ODN可以有效促进DC的功能成熟,可应用于科研及临床中。 Objective To improve the method of the amplification of dendritic cells（DCs） and to observe the stimulatory effect of CpG-ODN on the maturation of the murine bone marrow-derived dendritic cells. Methods Mice bone marrow-derived DCs were induced in RPMI-1640 with 10% FCS, 500u/ml rm-GM-CSF and 200u/ml rmIL-4. The stimulatory effect of CpG-ODN on the maturation of DCs was evaluated by morphology, phenotype and alloreactivity of DCs. Results A large number of DCs could be collected after 10d. The stimulatory effect of CpG-ODN on the maturation of DCs was confirmed with detecting the expression of molecules on the surface of DCs by FACS and MLR. Conclusion CpG-ODN can effectively enhance the functional maturation of DCs. This method can be used in research and clinic,
作者 聂志林 靳风烁 梁培禾 叶锦
机构地区 第三军医大学大坪医院野战外科研究所泌尿外科
出处 《重庆医学》 CAS CSCD 2008年第2期136-138,共3页 Chongqing medicine
关键词 树突状细胞 寡核苷酸 佐剂 小鼠 dendritic cell oligodeoxynucleotide adjuvants mouse
分类号 R392 [医药卫生—免疫学]
  • 相关文献

参考文献10

  • 1Son YI,Egawa S,Tatsumi T,et al.A novle bulk culture method for generating mature dendritic cells from mouse bone marrow cells[J].J Immunol Methods,2002,262(12):145.
  • 2Actins H,Davis BR,Kirby JA,et al.Polarisation of a T-helper cell immune response by activation of dendritic cells with CpG-containing oligonucleotides:a potential therapeutic regime:for bladder cancer immunotherapy[J].Br J Cancer,2003,89(12):2312.
  • 3Smaroula D,Julius MC,Robert L.Function of CD80 and CD86 on monocyte and stem cell derived dendritic cells[J].Exp Mol Pathol,2003,75:217.
  • 4Gansuvd B,Hagihara M,Higuchi A,et al.Umbilical cord blood dendritic cells are a rich source of soluble HLA-DR:synergistic effect of exosomes and dendritic cells on autologous or allogeneic T-Cell proliferation[J].Hum Immunol,2003,64(4):427.
  • 5Mahyar NS,Jacques B,Joseph F,et al.Dendritic cell based tumor vaccines[J].Immunol Lett,2000,74(1):5.
  • 6Karsho T,Akira S.Dendritic cell function in Toll-like receptor and Myd88-knockout mice[J].Trends Immunol,2001,22(1):78.
  • 7Pilon TS,Li W,Briggs JJ,et al.Immunostimulatory effects of CpG-ODN upon dendritic cell-based immunotherapy in a murine melanoma model[J].J Immunother,2006,29(4):381.
  • 8Bevaart L,Van Ojik HH,Sun AW,et al.CpG oligodeoxynucleotides enhance FcgammaRI-mediated cross presentation by dendritic cells[J].Inter Immunology,2004,16(8):1091.
  • 9Ninalga C,Loskog A,Klevenfeldt M,et al.CpG oligonucleotide therapy cures subcutaneous and orthotopic tumors and evokes protective immunity in murine bladder cancer[J].J Immunother,2005,28(1):20.
  • 10Kim TG,Kim CH,Won EH,et al.CpG-ODN-stimulated dendritic cells act as a potent adjuvant for E7 protein delivery to induce antigen-specific antitumour immunity in a HPV 16 E7-associated animal tumour model[J].Immunology,2004,112(1):117.

同被引文献16

  • 1杜宇琛,林苹,张洁,陆燕蓉,宁其志.CpG ODN加强树突状细胞疫苗抗Lewis肺癌的研究[J].中华肿瘤杂志,2005,27(1):1-5. 被引量：8
  • 2Krieg AM, Love-Homan L,Yi AK,et al. CpG DNA induces sustanined IL-12 expression in vivo and resistance to listeria monobytogenes challenge[J]. J Immunol, 1998, 161(5) :2428.
  • 3Pilon TS, Li W, Briggs JJ, et al. Immunostimulatory effects of CpG-ODN upon dendritic cell-based immuno- therapy in a murine melanoma model[J]. J Immunother, 2006,29(4) :381.
  • 4Actins H,Davis BR,Kirby JA,et al. Polarisation of a T- helper cell immune response by activation of dendritic cells with CpG-eontaining oligonucleotides: a potentiao therapeutic regime: for bladder cancer immunotherapy [J]. Br J Cancer,2003,89(12) :2312.
  • 5Silke G,Michael S, Herbert R. Dendritic cells pulsed with tumor derived lysate augment T cell mediated tumor cell lysis[J]. Otolaryngol Head Neck Surg, 2004, 131 (2): 176.
  • 6Kono K,Takasha A,Sugai H, et al. Dendritic ceils pulsed with HER-2/neu derived peptides can induce specific cell responses in patient with gastric cancer[J]. Clin Cancer Res,2002,8(11) :3394.
  • 7Nair SK, Morse M, Boczkowski D, et al. Induction of tumor specific cytotoxic T lymphocytes in cacer patients by antologous tumor RNA transfected dendritic cells[J]. Ann Surg,2002,235(4) :540.
  • 8Krieg AM. Mechanism and application of immune stimu- latory CpG oligodeoxynucleotides [J]. Biochem Biophys Acta, 1999,1489 (1) : 107.
  • 9Caux C, Massacrier C, Dubois B, et al. Respective involvement of TGF-βand IL-4 in the development of Langerhans cells and non-Langerhans dendritic cells from CD34 + progenitors[J]. J Leukoe Biol, 1999,66(5) :781.
  • 10Pilon TS, i.i W, Briggs JJ, et al. Immunostimulatory effects of CpG-ODN upon dendritic cell-based immunotherapy in a murine melanoma model[J]. J Immunother, 2006,29(4) :381.

引证文献2

二级引证文献3

重庆医学
2008年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部