摘要
[目的]为研制和筛选猪细小病毒核酸疫苗提供依据。[方法]对猪细小病毒Vaccine株VP2进行克隆、测序以及抗原性分析。[结果]参考GenBank公布的NADL-2株序列，设计出1对引物，并利用该引物扩增了猪细小病毒vaccine株VP2主要抗原基因，将其克隆到pGEM-T Easv载体上，测序获得656 bp核苷酸序列，并推导出其氨基酸序列，共编码218氨基酸，与NADL-2株相应的序列进行比较分析，比NADL-2毒株缺失781 bp，两者核苷酸同源性为98，9％，氨基酸同源性为97，2％；应用DNAStar软件对氨基酸的抗原表位进行了预测，共有7个抗原表位，分别在氨基酸N端的第10～18、34～39、94～103、121～126、137～144、156～165和209～214区段，此序列具有较好的免疫原性、[结论]该研究为发展特异性和敏感性诊断系统和研制疫苗提供了有力的技术依据，为研究蛋白质特性分析提供了理论依据。
The objective of the study is to provide basis for the development and screening of parcine parvovirus nucleic acid vaccine, Designing a pair of primers according to NADL - 2 strain of PPV from Gen Bank, the major antigen regions of VP2 of vaccine strain were amplified by PCR. The PCR products were cloned into pGEM - T Easy vector. 656 bp nucleotide sequences were acquired; and 218 bp amino acid sequences were deduced. Compared it with NADL- 2 strain comespandingly, absence 781 bp and the homologies of nucleotide and amino acid sequences of the two strains were 98.9% and 97.2% respectively. The prediction of VP2 antigen epitopes sbowed that seven antigen epitupes were located at the segroents of 10 - 18,34 - 39,94 - 103, 121 - 126,137 - 144,156 - 165 and 209 - 214 in amino acid N terminal. This study has provided technical basis for developing specific and sensitive diagnosis system and developing vaccines and provided theoretic basis for study on protein property analysis.
出处
《安徽农业科学》
CAS
北大核心
2008年第1期37-39,共3页
Journal of Anhui Agricultural Sciences
