摘要
目的动态观察肺部铜绿假单胞菌(PAE)感染前后肺组织角质细胞生长因子(KGF)及支气管肺泡灌洗液(BALF)中KGF及表面活性蛋白A(SPA)的变化,并探讨其意义。方法选择SPF级SD大鼠,每点6只,采用PAE标准菌株ATCC27853经气管注入的方式建立肺部感染模型,在感染建立之前(0h)、感染建立之后6、9、72 h等4个时间点收集BALF和肺组织标本,用Western印迹检测肺组织KGF蛋白表达,采用斑点印迹法测定BALF中SPA蛋白含量。结果感染铜绿假单胞菌后,肺组织KGF呈随时间递增趋势,到72 h时达到高峰;感染后72 h肺组织的KGF蛋白量显著高于感染前(P<0.05);而BALF中SPA在肺部感染后呈先减少再增加的趋势;6 h时降低后开始上升,到72 h时达到高峰;6 h点低于感染前(P<0.05),而到72 h点已经高于感染前水平(P<0.05)。结论KGF和SPA均表现为感染初期相对不足;KGF在感染后的逐步增高可能是SPA最终上升的重要因素;肺部感染初期是KGF/SPA补充替代治疗的最佳时机。
OBJECTIVE To observe a time course of keratinocyte growth factor （KGF） and surfactant protein A （SPA） changes in rats with Pseudomonas aeruginosa pneumonia and to find out the significances of them. METHODS Specific pathogen-free male Sprague-Dawley rats were used to study. Standard P. aeruginosa strain ATCC 27853 was instilled into airway by the tracheal route to induce model of pneumonia. Samples of lung tissue and BALF were harvested before infection, and 6 h, 9 h and 72 h after infection. Six rats per each point were sacrificed for harvesting samples. Expression of KGF protein in lungs was detected by Western blotting. Western- dot-blot was used to detect SPA expressions in BALF. RESULTS After infected with P. aeruginosa, KGF protein in lungs was markedly increased, reached the peak at 72 h postinfection. KGF protein level at 72 h postinfection was markedly higher than that of before infection （P〈0. 05）. SPA in BALF began to decrease till 6 h after infection, followed by a continuous increase, reaching the peak at 72 h postinfection. With the value before infection, the peak value of SPA was higher than the value before infection （P〈0. 05）. CONCLUSIONS There are relative deficit of KGF and SPA expression in infected rats at the early stage of pneumonia. The increase in KGF contributes to the change in SPA after infection. The early stage of pneumonia may be the right juncture for KGF/ SPA vicarious therapy.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2008年第1期22-24,共3页
Chinese Journal of Nosocomiology
基金
上海市卫生系统优秀学科带头人培养计划资助项目(98BR030)
