摘要
【目的】寻找更高活性的芳香二脒类抑菌化合物。【方法】以对氰基苯酚、1，3-二溴丙烷和1，4-二溴丁烷为原料，合成了A3、B3、B4、C3、C4、D3、D4和E38个丙烷脒衍生物，用1H—NMR和MS鉴定了其结构，用生长速率法和黄瓜真叶法测定了其活性，并与丙烷脒进行了比较。【结果】生长速率法测试表明，8种丙烷脒衍生物对番茄灰霉病菌（Botrytiscinerea）菌丝生长均具有较强的抑制作用，其中N，N-二正丙基丙烷脒（B3）、N，N-二正丙基丁烷脒（B4）、N，N-二异丙基丙烷脒（C3）、N，N-二异丁基丁烷脒（D3）对番茄灰霉病菌生长抑制的毒力均高于丙烷脒，其EC50为0．3043～3．4730mg／L；黄瓜真叶法测定表明，50mg／L剂量下B3、B4、C3的3d保护效果均在60％以上，优于丙烷脒处理的57．55％；B3、B4的3d治疗效果分别为71．70％和68．87％，与丙烷脒防效（64．15％）相当。【结论】B3、134较丙烷脒抑菌活性有较大提高，有望成为丙烷脒产品的更新品种。
[Objective] The research is to find more compounds with antifungal activity. [Method] Eight propenaindine analogs A3,B3,B4,C3,C4,D3 ,D4 and E3 were synthesized from 4-cyanophenol and a,ω-dibromopane. All compounds were confirmed by 1H-NMR and MS. The inhibitory effects were studied with the mycelial growth rate method. [Result] The results showed that four compounds such as N, N-bispropy-lpropamidine （B3）, N, N-bispropylbutylamidine （ B4 ）, N, N-diisopropylpropamidine （ C3 ）, N, N,-disisobu- tylbuytlamidine （D3）, had high inhibitory effect on the growth of Botrytis cinerea, with ECs0 ranging from 0.241 8 mg/L to 3. 473 0 mg/L, higher than propamidine;the protective and therapeutical efficacy of B3,B4 and C3 on leaves of cucumber all higher than that of propamidine. [Conclusion] B3 and B4 own more potent activity than propamidine and can be used to substitute propamidine.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2008年第1期168-172,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家“十五”科技攻关重大专项(2002BA516A04)
西北农林科技大学科研专项
关键词
丙烷脒
芳香二脒类
番茄灰霉病菌
抑菌活性
propamidine
aromatic diamidino
Botrytis cinerea
Anti-bacterial activity
