摘要
目的:分析乙型肝炎患者血清病毒DNA(HBV-DNA)与前S1抗原(PreS1)及e抗原(HBeAg)的关系,评价PreS1的临床检测意义。方法:筛选179例实时荧光定量PCR HBV-DNA水平阳性患者血清,采用ELISA法检测PreS1和HBeAg。结果:179例HBV DNA阳性血清中,HBeAg总阳性率为66.5%,随着HBV-DNA水平的升高,HBeAg的检出率大幅升高。PreS1总阳性率为83.8%,在三组不同的HBV DNA水平中,1组与2组、2组与3组间无差别(P>0.05),仅1组与3组间存在一定的差异(χ2=9.38,P<0.05)。结论:PreS1与HBV-DNA密切相关,PreS1检测可反映其体内HBV病毒的复制情况,具有一定的临床价值。
Objective.. To study the relationship among HBV-DNA,PreS1 and HbeAg in Patients with Hepatitis,and evaluate the clinical significance of PreS1. Methods： Serum with positive level of HBV-DNA（〉 500 copies/mL） in 179 patients were selected and was detected by real-time fluorescence quantity PCR. Serum PreS1 and HBeAg were detected by ELISA. Results： In 179 patients serum, HBeAg of 119 patients（66.5 %） was positive. The detection rate of HBeAg was increased along with the increasing of HBV DNA. PreS1 of 150 patients（83.8%）was positive. Among three groups with different HBV DNA levels, there was no difference between group 1 and group 2,and as well as group 2 and group 3（P〉0.05）. Only between group 1 and group 3, there was a little difference（x^2 = 9.38, P〈0. 05）. Conclusion： There was a close relationship between HBV- DNA and PreS1. PreS1 has a diagnostic significance to reflect the replication and contagion of HBV.
出处
《河北北方学院学报（医学版）》
2008年第1期38-39,共2页
Journal of Hebei North University:Medical Edition
