摘要
目的观察杀草强对FRTL-5细胞中甲状腺球蛋白基因(tg)、甲状腺过氧化物酶基因(tpo)、钠碘转运体蛋白基因(nis)、促甲状腺激素受体基因(tshr)、甲状腺转录因子基因1(ttf-1)和双链复合蛋白8基因(pax-8)转录的影响,初步探索其干扰甲状腺激素活性的机制。方法0.001、0.01和0.1mg/ml杀草强分别处理FRTL-5细胞24h,收集细胞并提取细胞总RNA,用RT-PCR观察杀草强对tg、tpo、nis、tshr、pax-8和ttf-1基因转录的影响。结果杀草强各剂量组使tg基因转录均显著增强;杀草强高剂量组使pax-8和tshr基因转录显著降低,使ttf-1基因转录显著降低;杀草强各剂量组对tpo和nis基因无显著影响。结论杀草强干扰甲状腺激素活性可能与其对tg,tshr,pax-8和ttf-1基因的影响有关。
Objective To observe the effects of amitrole on the transcription of thyroglobulin （tg）, thyroid peroxidase （tpo）, Na^＋/I- symporter （n/s）, Na^＋/I- symporter （his）, thyroid-stimulating hormone receptor （tshr）, thyroid transcription factor 1 （ttf-1） and paired-domain protein-8 （pax-8） genes in FRTL-5 cells and investigate the mechanism of amitrole for intervening in thyroid hormone activity. Methods FRTL-5 cells were treated with amitrole at 0.001, 0.01 and 0.1 mg/ml for 24 h, respectively, after which the cells were collected for extraction of the total RNA. RT-PCR was used to examine the effects of amitrole on the transcription oftg, tpo, his, tshr, pox-8 and ttf-1 genes in FRTL-5 cells. Results Amitrole significantly induced tg gene transcription at all the doses, but produced no obvious effects on tpo and nis gene transcription. At the concentration of 0.1 mg/ml, amitrole significantly reduced pox-8 and tshr gene transcription but increased ttf-1 gene transcription. Conclusion The effects of amitrole on thyroid hormone activity may be related with its actions on tg, ttf-1, tshr and pox-8 gene transcription.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2008年第1期12-15,共4页
Journal of Southern Medical University
基金
十一五国家科技支撑计划重大项目(2006BAK02A07)~~
