摘要
本文分别用对硝基酚磷酸酯、3＇-[32p]标记的RNA和大肠杆菌噬菌体入DNA作底物测定了新疆产穴居狼蛛毒中与核酸代谢有关的酶,发现此毒中含有磷酸单酯酶、核糖核酸酶和脱氧核糖核酸酶。
Activities of phosphomonoesterase and nucleases hydrolysing RNA and DNA were presented in a solution of the crude venom from a spider (Lycosa sing-oriensis) collected in Xinjiang Region, China. The substrates for assaying these enzymes were 4-nitrophenyl phosphate disodium salt, 3' terminally 32p-labclled RNA and nonlabelled DNA respectively. After incubation of RNA and DNA with the venom solution, the products were detected by polyacryla-mide gel or agarose gel electrophoresis.
关键词
穴居狼蛛毒
磷酸单酯酶
核酸酶
spider venom, phosphomonoesterase, DNase, RNase
