摘要
通过显微注射法,将转基因栽体pBcA3EG及辅助质粒pA3H导入产后1h的蚕卵,在G_1代获得家蚕转基因的阳性个体。通过对G_2代转基因蚕基因组的Southern杂交分析表明,获得的该转基因品系为EGFP基因的单拷贝插入。同时对G_2代个体不同发育时期及不同组织进行荧光观察,发现EGFP在转基因家蚕幼虫期的表达较其它时期强,而幼虫时期强烈的EGFP主要由中肠组织的杯形细胞高量表达所致。这些结果初步表明A3启动子在家蚕的不同发育时期及不同组织存在表达的活性差异。
The piggyback vector pBcA3EG and the assistant plasmid pBcA3H were introduced into 1-hour-old eggs of silkworm, and transgenic silkworms were obtained in the G1 generation. Southern blotting analysis showed that the EGFP gene was inserted into the silkworm genome with a single copy from the G2 genome DNA. Fluorescent observation of the expression of EGFP in different development periods and different tissues of G2 silkworm showed that EGFP was expressed more strongly in the larval period than in the other periods, which was due to the high expression of EGFP in the calyciform cells of the midgut of the larvae. It is tentatively concluded from these results that the activity of Actin3 promoter may vary with different development periods and tissues of silkworm.
出处
《蚕学通讯》
2007年第3期1-6,共6页
Newsletter of Sericultural Science
基金
973计划(2005CB121000)
高等学校学科创新引智计划(B07045)
重庆市院士基金
