摘要
目的探讨视网膜母细胞瘤（ｒｅｔｉｎｏｂｌａｓｔｏｍａ，Ｒｂ）细胞的增殖活性与患者预后的关系。方法应用抗增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）单克隆抗体ＰＣ－１０，采用ＬＳＡＢ免疫组织化学方法及Ｐｌｏｔｏｎ银染技术，对４８例Ｒｂ石蜡标本进行ＰＣＮＡ和核仁组成区嗜银蛋白（ａｒｇｙｒｏｐｈｉｌｉｃｐｒｏｔｅｉｎｓｏｆｔｈｅｎｕｃｌｅａｒｏｒｇａｎｉｚｅｒｒｅｇｉｏｎｓ，ＡｇＮＯＲｓ）检则。结果Ｒｂ组织ＰＣＮＡ标记指数（ｌａｂｅｌｉｎｇｉｎｄｅｘ，ＬＩ）与其相对应组织切片的ＡｇＮＯＲｓ计数有显著相关性（ｒ＝０．６４７，Ｐ＜０．００１）。其中行单纯眼球摘除治疗并有５年随访资料的２９例Ｒｂ中，术后存活时间≥５年组（１９例）与术后存活时间＜５年组（１０例）比较，前组的Ｒｂ组织平均ＰＣＮＡＬＩ和平均每核ＡｇＮＯＲｓ计数均显著低于后组（Ｐ＜０．０１，Ｐ＜０．０５），表明ＰＣＮＡＬＩ或ＡｇＮＯＲｓ计数与Ｒｂ预后呈明显的负相关。
Objective To investigate the relationship between the proliferative activity of retinoblastoma (Rb) cells and the prognosis of the tumor. Methods Proliferating cell nuclear antigen (PCNA) and argyrophilic nucleolar organizer regions (AgNORs) in paraffin embedded Rb specimens from 48 cases were detected by LSAB immunohistochemical method using an anti PCNA monoclonal antibody and the technique of ploton. Results The PCNA labeling index (PCNALI) and the AgNORs counts were well correlated ( r =0.647, P <0.001). In 29 patients of the 48 cases receiving simple eyeball extirpation and being followed up for 5 years, the mean PCNALI and the mean numbers of AgNORs per nucleus in 19 patients surviving ≥5 years after operation were markedly lower than that in 10 patients surviving <5 years after operation ( P <0.01, P <0.05). Conclusion It is indicated that the PCNALI or the AgNOR count is negatively related with the clinical prognosis of Rb patients, and can be useful in judging the prognosis.
出处
《中华眼科杂志》
CSCD
北大核心
1997年第4期293-296,共4页
Chinese Journal of Ophthalmology
关键词
视网膜母细胞瘤
增殖细胞核抗原
AGNORS
眼肿瘤
Retinoblastoma
Proliferating cell nuclear antigen
Nucleolus organizer region
Immunohistochemistry
Prognosis
