摘要
应用胃泌素多克隆抗体和链霉菌抗生物素蛋白-过氧化物酶免疫组织化学方法(SP法),对中华乌塘鳢(Bostrichthys sinensis)、黄鳍鲷(Sparus latus)、牙鲆(Paralichthys olivaceus)、褐篮子鱼(Sigauns fusces-ens)和大弹涂鱼(Boleophthalmus pectinirostris)消化道胃泌素细胞(G细胞)进行免疫组化鉴别和定位。结果显示:中华乌塘鳢G细胞主要分布在小肠,直肠偶见。黄鳍鲷G细胞仅在小肠发现。牙鲆G细胞大量位于幽门胃和小肠。褐篮子鱼G细胞在幽门盲囊和小肠有少量分布。大弹涂鱼G细胞在贲门胃、幽门胃和小肠均有出现,大量分布于幽门胃。5种鱼类G细胞主要出现在胃和小肠的分布状况,与胃泌素调节胃肠消化吸收的功能相一致。5种鱼类G细胞形态类型多样,提示了胃泌素以不同的内分泌方式参与消化生理过程。
By using the gastrin antiserum and the immunohistochemical method of Streotavidin-Peroxid （S-P）, the investigation on the distributions of G cells in the digestive tract of Bostrichthys sinensis ,Sparus latus,Paralichthys olivaceus, Sigauns fuscesens and Boleophthalmus pectinirostris was made. The distributions of the G cells are as follows： In Bostrichthys sinensis, the G cells are mainly located in intestine, rarely in rectum In Sparus Zatus , the G cells were only observed in intestine, none in any other parts of the digestive tract In Paralichthys olivaceus, the G cells were observed numerously in stamachus pyloricus and intestine In Sigauns fuscesens, the G cells were distributed in pyloric caecum and intestine In Boleophthalmus pectinirostris, the G cells were observed in stomachus carsiacus, stamachus pyloricus and intestine, mainly located in stamachus pyloricus. The G cells were detected mainly in the stomach and intestine in 5 kinds of marine fish, the results show that the gastrin was correlated with function of digestion and absorption. The G cells were of polymorphism in 5 kinds of marine fish. It was suggested that the gastrin might exert the digestive physiology in different modes of endocrine.
出处
《海洋科学》
CAS
CSCD
北大核心
2008年第1期52-55,共4页
Marine Sciences
基金
国家自然科学基金资助项目(40276040)
福建省自然科学基金资助项目(B0210003)
