摘要
为探讨乳腺癌组织中ｐ５３基因丢失与ｐ５３ｍＲＮＡ表达的相关性，应用Ｓｏｕｔｈｅｒｎ杂交及反转录－定量ＰＣＲ技术对４７例乳腺癌组织进行了检测。结果发现：ｐ５３基因杂合性缺失率为３４．０％（１６／２４）；在乳腺正常腺体有中度ｐ５３ｍＲＮＡ表达，而在肿瘤组织中有４０．４％（１９／４７）患者伴ｐ５３ｍＲＮＡ高表达。ｐ５３基因缺失与ｐ５３ｍＲＮＡ高表达之间呈显著性相关（Ｐ＜０．０１）。提示：ｐ５３基因在人类乳腺癌形成和肿瘤演进过程中起重要作用。反转录一定量ＰＣＲ具有快速、简单、敏感、无放射性污染等优点，适于临床应用。
p53 gene deletion and p53 mRNA expression were examined in 47 cases of breast cancer by using Southern blotting and RT/PCR techniques. p53 gene was found to be partially deleted in 34.0% (16/47) of breast cancer tissues. In the normal breast gland, there was moderately p53 mRNA expression. In the tissues of carcinomas, 40.4% (19/47) of the cases were companied with p53 mRNA overexpression. A highly significant correlation between p53 gene deletion and p53 mRNA expression was found ( P<0.01) . The data indicate that p53 gene may play an important role in the occurrence and progression of breast cancer. The RT/PCR technique is a quick, simple and sensitive method and suitable for widely clinical use.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1997年第4期231-233,共3页
Chinese Journal of Medical Genetics
