摘要
周期型马来丝虫感染期幼虫(L_3)在三种含人卵巢粘液性囊腺癌细胞系(OMC_(685))的RPMI1640培养系统中均能蜕皮发育为L_4,幼虫最长存活66天,蜕皮率和完成蜕皮率可分别达57.1％和89.3％。在不含细胞系的培养液中,幼虫最长存活14天,基本上不蜕皮。本实验结果提示OMC_(685)细胞系可能产生某些有利于周期型马来丝虫L_3体外生存和发育的物质。
Development of periodic Brugia malayi larvae from the infective stage (L3) to the fourth stage (L4) was observed in three culture systems of RPMI1640 supplemented with 10% fetal bovine serum and a human ovary mucin cystadenocarcinoma (OMC685) continuouls cell line feeder layer. The best survival, growth and development were seen in the culture system with OMC686 cell line supplemented with glucose (1g/1): the larvae survived for 66 days, the percentages of moulting and completed moulting were 57.1% and 89.3% the growth percentages of L4 in length and width were 216.4% and 194.9% respectively. In the media without OMC685 cell line the larvae survived only for 14 days, and none moulted. The results suggest that OMC685 cell line might produce some substances facilitating the survival and development of L3 of periodic B. malayi, and exert conspicuously some positive effect on the survival duration, moulting process and developmen of La. The study also confirms that glucose can promote the development of filaria L3 in vitro cultivation.
出处
《动物学报》
SCIE
CAS
CSCD
1989年第2期182-188,共7页
ACTA ZOOLOGICA SINICA
关键词
马来丝虫
卵巢癌细胞系
体外培养
Brugia malayi
Infective larvae (L3)
Cultivation in vitro
Moult
Human ovary mucin cystadenoearcinoma cell line (OMC685).
