摘要
用分子生物学的实验方法对非胰岛素依赖型糖尿病（NIDDM）患者的胰岛素基因进行检测。从NIDDM患者外周血的白细胞中提取DNA，以胰岛素基因5′-末端上游的某一DNA序列为引物，对特定的靶序列进行体外扩增，即TaqDNA聚合酶链式反应（PCR），将其扩增产物进行电泳分离，在紫外分析仪下观察结果并与正常人对照，发现16例NIDDM患者的胰岛素基因有所改变，提示胰岛素基因改变可能是导致NIDDM的原因之一。
DNA was extracted from the white blood cells of the patients with non-insulin-dependent diabetes mellitus,(NIDDM), using some DNA sequence of insulin gene 5'-end upstream as primer amplifying the sequence of special target, that is, Taq DNA polymerase chain reaction (PCR). Electrophoretic Segregation took place in the amplified substances.The result shows that under the ultroviolet lamp almost 16 patiens with NIDDM represented abnormal straps. The changes of insulin gene in the patients with this type of diabetes may result in one of NIDDM hereditary factors.
出处
《中国优生与遗传杂志》
1997年第4期12-13,共2页
Chinese Journal of Birth Health & Heredity
关键词
糖尿病
胰岛素基因
多基因遗传病
Non-insulin-dependent diabetes millitus (NIDDM), Insulin gene, Multigenerational hereditary disease
