摘要
目的探讨腹腔镜肾癌根治术前后患者外周血Fas/FasL表达及意义。方法对限期行肾肿瘤的患者均衡分成两组,腹腔镜组(LN)和开放手术组(ON)共52例。手术前、术后1 d、3 d、5 d,Fas/FasL免疫指标的变化进行检测,并对改变程度进行比较。结果两组手术均获成功,两组术中术后无明显并发症。两组术中失血、用止痛剂与手术后住院天数存在显著差异。FasL(LN组与ON组)均高于正常参考值,Fas(术后5 d)、FasL(术后1 d、5 d)、腹腔镜组与开放手术组间存在统计学差异(以α=0.05为检验水准)。结论外周血Fas/FasL表达与肿瘤发生发展、创伤程度有关,腹腔镜肾癌根治术低表达,开放手术高表达,腹腔镜肾癌根治术对机体免疫影响小;肿瘤患者均处于高表达,手术后表达下降,说明机体免疫功能得到恢复。
Objective The expression and significance of Fas and FasL on peripheral blood cell of patients with renal cell carcinoma （RCC） undergone either laparoscopic radical nephrectomy or open radical nephrectomy. Methods 52 patients with renal cell carcinoma admitted to Changzheng Hospital were balanced and allocated randomly into two groups. Cellular immune function and changes were studied in groups of patients undergoing either laparoscopic radical nephrectomy （LN） or open radical nephrectomy （ON）. Fas/FasL were measured by using flow cytometry. The tests being peri-operation and 1 d,3 d,5 d after operations The statistical methods were repeated-measured ANOVA T test and χ^2 test. If P〈 0.05, the difference is significant. Results Operations in both groups were successful with no obvious complications. Those in LN group, the number of patients using pain-killer, hospital stay and blood loss were less considerably than ON group. After operations Fas/FasL had significant changes in both groups （P〈0.05）. Conclusion The aberration of Fas/FasL is related with thedegree of trauma, occurrence and development of renal cell carcinoma. Comparing with normal datum, the expression of Fasl in RCC; is high. The expression of Fas/FasL in RCC; of ON group is much higher than that of LN group after-s. The immune response is less pronounced after a laparoscopic procedure as compared to a conventional approach in patients with renal cell carcinoma.
出处
《同济大学学报（医学版）》
CAS
2007年第6期38-40,48,共4页
Journal of Tongji University(Medical Science)
