摘要
对海参肠道β-1，3-葡聚糖酶的提取条件及酶学性质进行了研究．结果表明，最佳提取条件为：pH6．0的磷酸盐浸提液0．05mol／L，NaCl浓度为0．05mol／L，缓冲液体积与海参肠质量之比为4：1，硫酸铵饱和度为80％；该酶最适反应条件为：温度40℃，pH6．0；该酶在10-40℃，pH5．O～6．5酶活力稳定．Mn^2＋对酶具有一定的激活作用，Cu^2＋、Cd^2＋、Mg^2＋、Fe^2＋、Zn^2＋、Bd^2＋、K^＋、Ag^＋对酶存在不同程度的抑制，其中Cu^2＋对酶的抑制作用最强．
Extraction and properties ofβ-1,3-glucanase from the gut of sea cucumber were investigated in this paper. The optimum extraction condition was obtained as follows, phosphate buffer （pH 6.0, 0.05 mol/L, 0.05 mol/L NaCl）- gut=4 ： 1,saturation of （NH4）2SO4 was 80% The optimum temperature and pH of the enzyme were 40 ℃ and pH 6. 0. The crude β-1,3-glucanase was stable at pH 5.0-6.5. The enzyme was activated by 0.05 mol/L Mn^2＋ and inhibited by Cu^2＋ , Ca^2＋ , Mn^2＋ , Mg^2＋ , Fe^2＋ , Zn^2＋ , Ba^2＋ , K^＋ and Ag^＋ , in which Cu^2＋ was strongest inhibitor.
出处
《大连轻工业学院学报》
2007年第4期289-293,共5页
Journal of Dalian Institute of Light Industry
基金
国家自然科学基金资助项目(30571449)
科技部国际重大合作项目(2006DFA32580)
国家重点基础研究发展计划(973)资助项目(2006CB708210)
