摘要
目的：观察分析烧伤创面愈合过程中Ｔ淋巴细胞及其亚群的分布、动态变化及其意义。方法：Ｗｉｓｔａｒ大鼠制成８％ＴＢＳＡⅡ度烧伤创面，于伤前及伤后第１，３，５，７，１０和１４天作动态创面活检，冰冻切片作Ｔ细胞及其亚群的免疫组化染色。记录染色切片每平方毫米阳性细胞数。结果：（１）伤后第３天即有Ｔ细胞浸润于创南，第７天达峰值，继之逐渐下降；（２）创面ＴＨ／ＴＳ的比值约为２∶１，较为稳定；（３）Ｔ细胞主要分布于真皮网状层。结论：烧伤创面愈合过程中Ｔ细胞及其亚群有其变化的特征性Ｔ细胞及其亚群可能参与对烧伤创面愈合的调控。
Objective:To study the distribution and dynamic changes of T cells and their subsets in partial thickness burn wound healing. Methods:Wistar rats receiving 8%TBSAⅡ degree burn injury were biopsied 1,3, 5, 7, 10 and 14 d respectively postinjury. The wound tissues sections were stained using immunoperoxidase techniques with monocolonal antibody to T cells and their subsets (T H and T S) and with HE. Then positive stained cell counts per square milimetre in each section were recorded.Results:(1)T cells could be observed 3 d postburn and reached their peak 7 d postburn.(2)2∶1 of T H to T S ratio was noted during these days. (3)Most of T cells were scattered among the dermal reticulum of burn wound.Conclusion:These results indicate that T cells and their subsets may play an important role in tissue repair of burn wound.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1997年第2期157-159,共3页
Academic Journal of Second Military Medical University
