摘要
目的：建立简便有效、适用于药动学研究的法莫替丁血药浓度高效液相（ＨＰＬＣ）测定方法。方法：采用柱切换ＨＰＬＣ技术。分析柱（１５０ｍｍ×４．６ｍｍ，ＩＤ）固定相为ＹＷＧＣ１８（５μｍ），分析流动相为甲醇与乙酸铵缓冲液；预处理柱（５０ｍｍ×４．６ｍｍ，ＩＤ）填料为ＹＷＧＣ１８（１０μｍ），预处理流动相为乙酸铵缓冲液。结果：法莫替丁血浆浓度标准曲线线性范围为５～２００ｎｇ／ｍｌ（ｒ＝０．９９９８），血浆中最低检测质量浓度为４ｎｇ／ｍｌ。方法日内及日间精密度分别为２．９７％±１．５６％，６．０４％±１．６２％，回收率为９８．３７％±２．９１％。结论：应用本方法研究法莫替丁在正常人体内的药动学，具有简便迅速、准确灵敏、易于实现自动化等特点。
Objective:To establish a simple and effective HPLC method for determination of famotidine in plasma for pharmacokinetic study. Methods: A HPLC method with column switching technique was used. The analytical column(150 mm×4.6 mm,ID)was packed with YWG C 18 (5 μm)and the pretreating column(50 mm×4.6 mm,ID) was packed with YWG C 18 (10 μm); A solution of 0.2 mol/L ammonium acetate (adjusted pH to 3.04 with sulfuric acid) was used as pretreating mobile phase and 14%(V/V) methol in it was used as analytical mobile phase. The wave length was 266 nm. Results: The method was linear with the plasma concentration range of 5 ～200 ng/ml(r=0.9998), and the detection limit was 4 ng/ml.The precision was 2.97%±1.56% for within day and 6.04%±1.62% for between days respectively. The relative recovery of the method was 98.37%±2.91% . [WT5HZ]Conclusion:The method is easily to be applicated automatedly as it is simple, rapid, accurate and sensitive.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1997年第2期141-143,共3页
Academic Journal of Second Military Medical University
基金
全军"八五"规划科研基金
