摘要
目的研究采用高效液相法测定麻黄中盐酸麻黄碱的含量，以提高该药的质控水平。方法Agilent Zorbax—C18柱；乙腈-0．025mol·ml^-1磷酸二氢钠溶液（磷酸调凭pH值3．0）（5：95）为流动相；检测波长为206nm；柱温30℃；流速1ml·min^-1。结果线性范围92．81～773．40ng；平均回收率为99．9％，RSD为（n=6）。结论方法简便、准确、灵敏，重现性好。
Aim To establish a method of RP-HPLC for determination of ephedrine hydrochloride in ganmao capsule,to promote the quality control of this medicine. Methods Agilent zorbax-Cls column was used. The mobile phase was acetonitrile-0. 025 mol · ml^-1 sodium dihydrogen phosphate（ pH value adjusted to 3.0 with phosphoric acid） （ 5： 95 ）. Detection wavelength was at 206 nm. Column temperature was 30℃ and the flow rate is 1 ml ·min^- 1. Results The linear range was between 92.81 and 773.40 ng. The average recovery rate of ephedrine hydrochloride was 99.9% and RSD was 0.8 （n = 6）. Conclusion This method is simple,accurate,sensitive and reproducible.
出处
《安徽医药》
CAS
2007年第12期1090-1091,共2页
Anhui Medical and Pharmaceutical Journal
