摘要
目的了解临床分离阴沟肠杆菌中qnrB和qnrS基因的分布。方法PCR检测qnrB和qnrS基因并将阳性扩增产物进行测序分析;纸片扩散法测定qnrB和qnrS基因阳性菌株对10种抗菌药物的敏感性。结果81株阴沟肠杆菌中,5株qnrB4基因阳性(6.5%)、2株qnrS1基因阳性(2.5%);qnr基因阳性菌株对氯霉素、阿米卡星、头孢西丁、头孢噻肟等显示多重耐药,其中3株对环丙沙星耐药。结论临床分离阴沟肠杆菌中存在qnrB和qnrS基因阳性菌株,为多重耐药菌,临床应加强检测和监测。
Objective To investigate the prevalence of qnrB and qnrS genes in Enterobacter cloacae isolates. Methods qnrB and qnrS genes were identified by PCR and sequencing. Susceptibility of E. cloacae isolates with qnr genes to fluoroquinolones and other antimicrobial agents were tested by disk diffusion method. Results Of the 81 E. cloacae isolates, qnrB4 and qnrS1 genes were identified in 5 （6.5 % ） and 2 （2. 5 % ） isolates, respectively. Those isolates with qnr genes were resistant to chloramphenicol, amikacin, cefoxitin, cefotaxime, and 3 of 7 were resistant to ciprofloxacin. Conclusions qnrB and qnrS genes were prevalent in Enterobacter cloacae isolates with resistance to most of antimicrobial agents. More attention should be paid to the surveillance of such strains.
出处
《中国感染与化疗杂志》
CAS
2007年第6期456-458,共3页
Chinese Journal of Infection and Chemotherapy
基金
安徽省自然科学基金资助项目(050430804)
安徽省高等学校省级自然科学研究基金资助项目(kj2007B145)
中国博士后科学基金资助项目(20060400202)
安徽省博士后科学基金资助项目(2006-8)
关键词
阴沟肠杆菌
QNR基因
耐药性
Enterobacter cloacae
qnr gene
Antibiotic resistance
