摘要
目的:观察复方白龙片(白龙),对人胃癌细胞内两条信号通路共调作用。方法:分别测定不同状况下人胃癌MGc80-3细胞内cAMP水平、二酰基甘油(DAG)含量、蛋白激酶A(PKA)和蛋白激酶C(PKC)活性。结果:白龙处理MGc80-3细胞3h后,cAMP水平和PKA活性分别较对照组升高112.7%和19.7%,而DAG含量与PKC活性分别下降47.0%和64.2%;如用GF-109203X阻断PKC通路时发现cAMP水平与PKA活性分别升高78.9%和33.5%,而DAG含量与PKC活性则降低40.3%和56.3%;若白龙处理同时加PKA抑制剂则较白龙单纯处理时cAMP水平下降46.4%,PKA活性下降28.9%,而DAG含量与PKC活性分别升高50.7%与54.5%。结论:(1)白龙对MGc80-3细胞的增殖调控和两条信号系统之间具有因果关系。(2)该结果与环六亚甲基双乙酰胺对MGc80-3细胞信号系统的影响完全相似,反映出信号系统对肿瘤细胞交叉共调作用可能是中西医药对肿瘤细胞调控的共同物质基础。
Objective:To evaluate the effects of traditional Chinese medicine formulae(Bailong)on cAMP/PKA and dia-cylaglycerol(DAG)protein kinase(PKC)pathways of the MGc80-3cells.Methods:cAMP level,DAG content and activities of PKA and PKC were measured in different groups:(1)control,(2)1.8 mg/ml Bailong,(3)1.8 mg/ml Bailong+20ug/ml PKA inhibitor,(4)5μmal/L PKC inhibitor.Results:When MGc80-3 cells were in treatment with Bailong for 3 hours,cAMP level and PKA activity were 11.27%,and 19.7%higher than that of the control,while DAG content and PKC activity were 47.0%and 64.2%lower than that of the control;When PKC pathway was blocked by PKC inhibitor GF-109203X,cAMP level and PKA activity were increased by 78.8%and 33.5%as against that of the control,while the DAG content and PKC activity were decreased by 40.3%nd 56.3%respectively.When MGc80-3cells were treated with Bailong,and at the same time,blocked PKA pathways by PKA inhibitor,cAMP level and PKA activity were decreased by 46.0%and 28.9%,on the other hand,DAG content and PKC activity were increased by 50.7%and 51.6%as against that of the Bailong group.Conctasions:(1)There was relationship of causes and result between differentiation of MGc80-3 cells and the signal pathways.(2)Results of this study were similar to that of hexamethylenebisacetamide(HMBA).It is shown that the two signal systems are the foundation of regulative effects of Chinese medicine formulae Bailong or HMBA on proliferation and differentiation in MGc80-3 cells.
作者
谷善青
梁云燕
樊立人
李宝元
王代树
GU Shan-qing;LIANG Yun-yan;FAN Li-ren(Laboratory of Cell biolo-gy,Oncology School of Beijing Medical Unitersity and Beijing Institute for Cancer Research,Beijing,100034)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1997年第7期404-407,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学资助课题(N0.39480026)
关键词
复方白龙片
信号系统
胃肿瘤
中医药疗法
traditional Chinese formula Bailong
signal system
gastric cancer
