摘要
目的:观察携带GAD65的永生化神经前体细胞移植到癫癎大鼠脑内后的存活、迁徒、分化及GAD65表达。方法:将稳定传代培养的携带GAD65的永生化神经前体细胞于癫癎大鼠首次癫癎发作后4周移植入海马,免疫组化方法观察移植细胞在脑内的存活、迁徒、分化及GAD65表达。结果:移植细胞在脑内存活,表现为SV40Tag阳性和GAD65阳性;可迁徒到同侧海马DG区门区、颗粒层及对侧海马;在海马DG区可分化为神经元和星形胶质细胞,以神经元为主,而在针道损伤修复部位仅分为星形胶质细胞。结论:携带GAD65的永生化神经前体细胞在移植入癫癎大鼠海马后能存活、表达GAD65,并据细胞所处的不同微环境向不同方向分化。
Objective： To observe survival, migration, differentiation and expression of GAD65 of immortalized neural progenitor cells carrying GAD65 gene （INPCs-GAD65） after transplanting into the hippocampus of an epileptic rat model. Methods： INPCs-GAD65 cells were transplanted into the right hippocampus of an epileptic rat model induced by pilocarpine. Then the survival, migration and differentiation were investigated with immunohistochemistry. Results： The transplanted cells were survived and were positive for SV40Tag and GAD65. They migrated to the dental gyri（DG）, granular layer of bilateral hippocampus. SV40 Tag-positive cells were noted to differentiate into neurons and astrocytes, but mainly into neurons in DG. In tissues around the needle passage, the transplants were only differentiated into astrocytes. Conclusion： INPCs-GAD65 cells can survive, migrate, differentiate directionaly and express GAD65 in the hippocampus after being transplanted into the epileptic rat model.
出处
《神经损伤与功能重建》
2007年第6期340-342,共3页
Neural Injury and Functional Reconstruction
基金
湖北省卫生厅科研基金项目(JXIA03)
