摘要
目的:应用TaqMan荧光定量技术,了解前列腺癌患者CYP1A2 IVS4+43A/G位点基因表型。方法:应用ABI Prism7300型荧光定量PCR仪,通过序列检测系统测定85例前列腺癌患者CYP1A2 IVS4+43A/G的各种基因型,并分析不同基因型患者与前列腺癌分化之间的相关性。结果:85例前列腺癌患者中CYP1A2 IVS4+43A/G位点基因表型为AA5例(5.9%),AG33例(38.8%),GG47例(55.3%)。且各基因表型与前列腺癌Gleason评分无明显相关性。结论:CYP1A2 IVS4+43A/G各基因表型与前列腺癌分化之间无明显相关性。
Objective： To investigate the genotype of the single nucleotide polymorphisms （SNP） of CYP1A2 IVS4 ＋ 43 A/G in patients with prostate cancer by TaqMan allelic discrimination. Methods： TaqMan SNP allelic discrimination was performed with the ABI 7300 system to detect the genotype of CYP1A2 IVS4 ＋43A/G in 85 patients with prostate cancer. Results： The genotypes of CYP1A2 IVS4 ＋43A/G in the patients were as follows： AA subtype in 5 cases（5.9% ） , AG in 33 （38.8%） , and GG in 47 （55.3%）. Each subtype of CYPIA2 IVS4 ＋ 43 A/G was not correlated with the Gleason score of prostate cancer. Conclusion： There was no significant correlation between SNP of CYP1A2 and the differentiation of prostate cancer.
出处
《中华男科学杂志》
CAS
CSCD
2007年第11期1002-1004,共3页
National Journal of Andrology
