摘要
丹参冠瘿组织高产株系选择和丹参酮的产生宋经元张荫麟祁建军吕桂兰（中国医学科学院中国协和医科大学药用植物研究所北京１０００９４）丹参（Ｓａｌｖｉａｍｉｌｔｉｏｒｒｈｉｚａ）是治疗心血管系统疾病的重要中草药。前文我们已报道了丹参冠瘿组织培养的部分研究结果...
Crown galls were induced by direct infection of sterile seedlings of Salvia miltiorrhiza with Agrobacterium tumefaciens C58 and subcultured in 67 V hormone free medium by successively selecting red cell aggregates. A high tanshinone producing crown gall strain C1 was obtained after 12 months in subculture. It grows well and keeps high tanshinone producing characteristic in liquid stationary culture. It was obvious that yeast extract and Armillaria mellea as elicitors could promote the strain C1 to produce tanshinone. Tanshinone content of the strain C1 cultures was over three times higher than that of the crude drugs. The results indicated that the crown gall tissue and elicitor technique could be provided some new clues for producing tanshinone under the condition of liquid stationary culture.
出处
《生物工程学报》
CAS
CSCD
北大核心
1997年第3期317-319,共3页
Chinese Journal of Biotechnology
基金
中国医学科学院基金
中国协和医科大学科学基金
关键词
丹参
冠瘿组织高产系
选择
丹参酮
药用植物
High tanshinone producing crown gall strain, liquid stationary culture, elicitor
